Sequences from confirmation amplicon


Confirmation sequences for line 332B10 and hit At1g75450 are shown.
Line ID 332B10
Locus At1g75450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28316457
Primers used for PCR MI08 - 8474, show primer details
   
Sequence Name 18-K018322-022-332-B10C-MI08
GenBank Accession LN494107 [GenBank]
Sequencing primer gene specific primer, MI08: 5'-GATTACGTGGAAGGTTTTGTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K018322-022-332-B10C-MI08
GGACTCGTCAACAATTGGAGATCTTCTTTCTTCTCTCCACGTAACCCCGTCAAGATCTCC
TCTGTTAGTTCCAACGGCTCTGTTTTGTATTGCCTTGAGATCACCAAGAACTACCACGAC
TCCGACTCCGAAATCGTTGATCAGGTCACTTTCATTATTCACTTAGAAAAAAGCGATATT
TTCATTTTTTATATTGATGAATATCTGGAAGGATTTAACGCTATGCGACTATTGGGAAAT
CATTATGAAAAAATATTTAGTTTATATGATTGAAAGTGGTCTCCATAGTATTTTTGTTGT
GTCGACTTTATTATAACTTAAATTTGGAAGAGGACATGAAGAAGAAGCCAGAGAGGATCT
ACAGAGATCTAGCTTTTCCACCTGAACTTAATAATGCACATTTATATAATTATTTTTCTT
CTTCTAAAGTTTAGTTTATCACTAGCGAATTAATCATGGTTACTAATTAAGTAGTGGACA
GGGTCATGGACCACTCACTCACCAAATAATGATTCCTCTTTACTCTTAATGGACAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
   
Sequence Name 22-K018322-022-332-B10C-8409
GenBank Accession LN495908 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K018322-022-332-B10C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCCATTAAGAGTAAAG
AGGAATCATTATTTGGTGAGTGAGTGGTCCATGACCCTGTCCACTACTTAATTAGTAACC
ATGATTAATTCGCTAGTGATAAACTAAACTTTAGAAGAAGAAAAATAATTATATAAATGT
GCATTATTAAGTTCAGGTGGAAAAGCTAGATCTCTGTAGATCCTCTCTGGCTTCTTCTTC
ATGTCCTCTTCCAAATTTAAGTTATAATAAAGTCGACACAACAAAAATACTATGGAGACC
ACTTTCAATCATATAAACTAAATATTTTTTCATAATGATTTCCCAATAGTCGCATAGCGT
TAAATCCTTCCAGATATTCATCAATATAAAAAATGAAAATATCGCTTTTTTCTAAGTGAA
TAATGAAAGTGACCTGATCAACGATTTCGGAGTCGGAGTCGTGGTAGTTCTTGGTGATCT
CAAGGCAATACAAAACAGAGCCGTTGGAACTAACAGAGGAGATCTTGACGGGGTTACGTG
GAGAGAAGAAAGAAGATCTCCAATTGTTGACGAGTCCTTCGTCCACAATCACAAAA

Last Updated on 06.12.2018 16:11