Sequences from confirmation amplicon


Confirmation sequences for line 333F04 and hit At5g63390 are shown.
Line ID 333F04
Locus At5g63390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25392833
Primers used for PCR PH04 - 8474, show primer details
   
Sequence Name 47-K017463-022-333-F04C-PH04
GenBank Accession LN506037 [GenBank]
Sequencing primer gene specific primer, PH04: 5'-ATGAGAAGAAGGAAAAACTGTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K017463-022-333-F04C-PH04
AGTTTGGTGATGGTGGTTTAGGTATCGGGGACTTGATTTCTTCAGGGGAGTGAAGAAGAG
GAGAGAGAGATGGAAGAATGGAGATGGCGGATGCGAAGTAAAGAATGGATTTTTGATAGT
TCTCATCGATTTCGCCATTGAAGCAGCTTCTTTCGATCGTTGAAGAAAGTTTCTCAGTTG
GTTCTCTGTGCAAAGAGAGAGATATGTGAGATTCAGTTACAGAGACTTCTGATATTCTTG
TTTTCCCGGGAAAATAAGAAGCCAAGTACGAAGTACTAACGAACCGAGAAACTTCAAATT
GCCCCCTGGTTTTATATCTTCTTATGAATAAGGACCGTAATTTAATTTTATTATAAGTTT
AGCCCCGAATCGGTGATTAGCAAAATGGTAATTTCTTTTATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
   
Sequence Name 48-K017463-022-333-F04C-8409
GenBank Accession LN506366 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K017463-022-333-F04C-8409
TTTCCCGGACTGAAGCCTCTTTACATTGAATAAAAGAAATTACCATTTTGCTAATCACCG
ATTCGGGGCTAAACTTATAATAAAATTAAATTACGGTCCTTATTCATAAGAAGATATAAA
ACCAGGGGGCAATTTGAAGTTTCTCGGTTCGTTAGTACTTCGTACTTGGCTTCTTATTTT
CCCGGGAAAACAAGAATATCAGAAGTCTCTGTAACTGAATCTCACATATCTCTCTCTTTG
CACAGAGAACCAACTGAGAAACTTTCTTCAACGATCGAAAGAAGCTGCTTCAATGGCGAA
ATCGATGAGAACTATCAAAAATCCATTCTTTACTTCGCATCCGCCATCTCCATTCTTCCA
TCTCTCTCTCCTCTTCTTCACTCCCCTGAAGAAATCAAGTCCCCGATACCTAAACCACCA
TCACCAAACTCGATGCCCACCTCCACAGT

Last Updated on 06.12.2018 16:11