Sequences from confirmation amplicon


Confirmation sequences for line 333G01 and hit At4g04360 are shown.
Line ID 333G01
Locus At4g04360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2133529
Primers used for PCR 17dv - 8474, show primer details
   
Sequence Name 22-K110124-0022-333-G01C-17dv
GenBank Accession LN496156 [GenBank]
Sequencing primer gene specific primer, 17dv: 5'-GATGATGCTTTTGTTCTAGCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K110124-0022-333-G01C-17dv
ATGGACATCAATCAAGAAGCGAATCTATGTGCCTGGGAAGATCCTCAACGTTATCCTAGA
CCGGACTAATGTCAAGGTCACTACAAAGTAGGATTTAGAAAGAAGAAACAATTACAAAAC
CTGTCAAATGAACAAAATTTTATCTCTATTGAGAGTTTCCATTATATAGAAAAAGATTCT
TTTAGAAGCAAAAGATCTGTTCTTAGATAACATCAGAAGCAACAGTTCCTTCTTTCCATC
CTCCTTGCCGAGCTCGAAGCTCCCACATGTAAGAAAGTCTTCTCTGTTCATCTAGAGCTG
CTTCTGCTTTCTCTCTTGCTAGCTCACATGTTTCCATCCCCATACTGCATTTATCAGCCT
CTTTCTGATACTGAGACGCCGCTTTCTTAGCATCTAGCAGCAACCTATCTGCACGCCATT
CGTCTTCTTGAGCTTGTGCTTCTCTTAGCTTCAGCTCTTCAGCTACCATTTCAGTAAAAC
TACTCTCGCTCTCTTCACTCCCTTCCTCGTGCCTCATGCAATCTTGGCAATGAAACAAGA
TCCAACCAAAATGCACGTGAAATCTGCGACGTTTTAGCATTCTTACTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTTCATTCAAAAATGTAGATGTCCG
   
Sequence Name 06-K110123-0022-333-G01C-8409
GenBank Accession LN488859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K110123-0022-333-G01C-8409
TCGGACTGAGCCTTTAGTAAGAATGCTAAAACGTCGCAGATTTCCGTGCATTTTGGTTGG
ATCTTGTTTCATTGCCAAGATTGCATGAGGCACGAGGAAGGGAGTGAAGAGAGCGAGAGT
AGTTTTACTGAAATGGTAGCTGAAGAGCTGAAGCTAAGAGAAGCACAAGCTCAAGAAGAC
GAATGGCGTGCAGATAGGTTGCTGCTAGATGCTAAGAAAGCGGCGTCTCAGTATCAGAAA
GAGGCTGATAAATGCAGTATGGGGATGGAAACATGTGAGCTAGCAAGAGAGAAAGCAGAA
GCAGCTCTAGATGAACAGAGAAGACTTTCTTACATGTGGGAGCTTCGAGCTCGGCAAGGA
GGATGGAAAGAAGGAACTGTTGCTTCTGATGTTATCTAAGAACAGATCTTTTGCTTCTAA
AAGAATCTTTTTCTATATAATGGAAACTCTCAATAGAGATAAAATTTTGTTCATTTGACA
GGTTTTGTAATTGTTTCTTCTTTCTAAATCCTACTTTGTAGTGACCTTGACATTAGTCCG
GTCTAGGATAACGTTGAGGATCTTCCCAGGCACATAGATTCGCTTCTTGATTGATTGTCC
ATCTAGATATTTCCTGAGTTTGTCGTCCTGTGAAGCTAGAACA
Primers used for PCR 25do - 8474, show primer details
   
Sequence Name 29-K110037-0022-333-G01C-25do
GenBank Accession LN499333 [GenBank]
Sequencing primer gene specific primer, 25do: 5'-GCTCCCACATGTAAGAAAGTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K110037-0022-333-G01C-25do
GCTTCTGCTTTCTCTCTTGCTAGCTCAATGTTTCCATCCCCATACTGCATTTATCAGCCT
CTTTCTGATACTGAGACGCCGCTTTCTTAGCATCTAGCAGCAACCTATCTGCACGCCATT
CGTCTTCTTGAGCTTGTGCTTCTCTTAGCTTCAGCTCTTCAGCTACCATTTCAGTAAAAC
TACTCTCGCTCTCTTCACTCCCTTCCTCGTGCCTCATGCAATCTTGGCAATGAAACAAGA
TCCAACCAAAATGCACGTGAAATCTGCGACGTTTTAGCATTCTTACTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATT
   
Sequence Name 13-K110036-0022-333-G01C-8409
GenBank Accession LN492085 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K110036-0022-333-G01C-8409
TTAGTAAGAATGCTAAAACGTCGCAGATTTCACGTGCATTTTGGCTTGATCTTGTTTCAT
TGCCAAGATTGCATGAGGCACGAGGAAGGGAGTGAAGAGAGCGAGAGTAGTTTTACTGAA
ATGGTAGCTGAAGAGCTGAAGCTAAGAGAAGCACAAGCTCAAGAAGACGAATGGCGTGCA
GATAGGTTGCTGCTAGATGCTAAGAAAGCGGCGTCTCAGTATCAGAAAGAGGCTGATAAA
TGCAGTATGGGGATGGAAACATGTGAGCTAGCAAGAGAGAAAGCAGAAGCAGCTCTAGAT
GAACAGAGAAGACTTTCTTACATGTGGGAGCAACCTATCTGCACGCCATTCGTCTTCTTG
AGCTTGTGCTTCTCTTAGCTTCTGCTCTTCAGCTACCATTTCAGTAAAACTACTCTCGCT
CTCTTCACTCCCTTCCTCGTGCCTCATGCAATCTTGGCAATGAAACAAGATCCAACCAAA
ATGCGCGTGAGATCTGCGACGTTTTAGCATT

Last Updated on 06.12.2018 16:11