Sequences from confirmation amplicon


Confirmation sequences for line 333G05 and hit At1g09440 are shown.
Line ID 333G05
Locus At1g09440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3047280
Primers used for PCR NK06 - 8474, show primer details
   
Sequence Name 35-K102592-0022-333-G05C-8409
GenBank Accession LN501785 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K102592-0022-333-G05C-8409
CCGGAATGAGCCATTTACATTGAATATATTCAAACCTTAAGTCTTCCCATCTTAAAACCC
CTGTGGTGTCAAAGGAGATCAAGGAAGTCAGGATCGAGCATGTTGTCTCAACGAGTAGTA
ACTTCGATCCTCAGGATGAAAACAACAATGAATCTGACAAATTCTTGCTTAATTTGGAAA
TGGAGAAGAACAGAGAGAATGGTTTGAGTAGTAGCCGGTCTGGTTCAGGCAAAGAAGGTT
ATTTATGTGTTGCAAACCGGACTACTTCATCATTGTATGAGATGGCTACACCATCACCAA
CAGCTCTTTCTGGTTTACCTG
   
Sequence Name 35-K102593-0022-333-G05C-NK06
GenBank Accession LN501786 [GenBank]
Sequencing primer gene specific primer, NK06: 5'-TCTGAGACCATAACTCACAAGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K102593-0022-333-G05C-NK06
GCACAGACTCCATTGACAACTCGCCTCGGTAAACAACTCCATAGCCTCCTTCACCAATCA
CATTTTCCTTTGAGAATCTGTTTGTTGCTATCTCAAGATCTCTCAGAGTAAACCAATGAC
CCCATCCAAGGTGTGACTCAGGTAAACCAGAAAGAGGTGATGGTGATGGTGTAGCCATCT
CATACAATGATGAAGTAGACCGGTTTGCAACACATAAATAACCTTCTTTGCCTGAACCAG
ACCGGCTACTACTCAAACCATTCTCTCTGTTCTTCTCCATTTCCAAATTAAGCAAGAATT
TGTCAGATTCATTGTTGTTTTCATCCTGAGGATCGAAGTTACTACTCGTTGAGACAACAT
GCTCGATCCTGACTTCCTTGATCTCCTTTGACACCACAGGGGTTTGTATGATGGGAAGAC
TTAAGGTTTTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCTACATTCACTCCAAAATGG

Last Updated on 06.12.2018 16:11