Sequences from confirmation amplicon


Confirmation sequences for line 334C11 and hit At1g43780 are shown.
Line ID 334C11
Locus At1g43780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16566883
Primers used for PCR 60ab - 8474, show primer details
   
Sequence Name 01-K106147-0022-334-C11C-60ab
GenBank Accession LN486585 [GenBank]
Sequencing primer gene specific primer, 60ab: 5'-TAAATCTTCCTGAGGTTCAAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K106147-0022-334-C11C-60ab
CGTATGAATGGTCTATGTGCAGCAGGTATAGTATAACTTGAACCATCCCTAATCTTAATC
TATATGTCATCATTTATTTGAACTCTTAAGGTTATGTTTCATCATCATAGCCTCTTGAAC
TACAAATACACTGACGGGAACGCCAACATGCTTCCAATTCTTAAAAGAATCGTGAAGAGC
AAAGTCCCTGTTTGGGTCTTCAGGTATATTGATTCCATTCTTTAGACCCTTAATTTCTTT
GTTCTTCTACTTTAGCAATCTGGTGGTAAACGTTTAAATAAATAAAAAGAAAAATTAATT
ACTCAGATATCAAAGAAAAGTATGTTTATTTTTAGAATATTATACATCTTTTTTTAATTA
CTCATATGTTTCTAGCATTTGTGGAATTTTACTCAGATGTCATACAAATTAGGTGTATTT
ACCATAACCTTACAAAACTTTTTTTTATTACTGCAGTGGAGATGAAGATTCTGTAATTCC
ACTTCTTGGTTCAAGAACTTTGGTAAAAGAACTTGCAGATGATCTCAACTTCAATACCAC
AGTTCCTTATGGAGCTTGGTTCGACAAAGGATATATTCAATTGCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 17-K106148-0022-334-C11C-8409
GenBank Accession LN493861 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K106148-0022-334-C11C-8409
TTACATTGAATATATCCTGCAATTGAATATATCCTTTGTCGAACCAAGCTCCATAAGGAA
CTGTGGTATTGAAGTTGAGATCATCTGCAAGTTCTTTTACCAAAGTTCTTGAACCAAGAA
GTGGAATTACAGAATCTTCATCTCCACTGCAGTAATAAAAAAAAGTTTTGTAAGGTTATG
GTAAATACACCTAATTTGTATGACATCTGAGTAAAATTCCACAAATGCTAGAAACATATG
AGTAATTAAAAAAAGATGTATAATATTCTAAAAATAAACATACTTTTCTTTGATATCTGA
GTAATTAATTTTTCTTTTTATTTATTTAAACGTTTACCACCAGATTGCTAAAGTAGAAGA
ACAAAGAAATTAAGGGTCTAAAGAATGGAATCAATATACCTGAAGACCCAAACAGGGACT
TTGCTCTTCACGATTCTTTTAAGAATTGGAAGCATGTTGGCGTTCCCGTCAGTGTATTTG
TAGTTCAAGAGGCTATGATGATGAAACATAACCTTAAGAGTTCAAATAAATGATGACATA
TAGATTAAGATTAGAGATGGTTCAAGTTATACTATACCTGCTGCACATAGACCATTCATA
CGGTAGTTTAGTTCGGTTAGCATGGAGAGCCTTTTGAAC

Last Updated on 06.12.2018 16:11