Sequences from confirmation amplicon


Confirmation sequences for line 334G04 and hit At4g25840 are shown.
Line ID 334G04
Locus At4g25840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13138736
Primers used for PCR 19ci - 8474, show primer details
   
Sequence Name 50-K107936-0022-334-G04C-19ci
GenBank Accession LN507190 [GenBank]
Sequencing primer gene specific primer, 19ci: 5'-GAATCTTATTGGAAGATCGGAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K107936-0022-334-G04C-19ci
TTACATACTTAACGACGCTGAGAATTGACGTGTGACGTGTATATTATACGTCACACTGAT
TTGGGCTTTTAAATTGATTGGGTCTTCTTAATTAGGCTTTATGGGCCATTTTGACGACCC
AAAATAGTTTTTATTAAAACAAATAAACTTTGGATTGCTAGTGTAATTACGAGTAATTTA
AGATAAAATTTTGGGAACTATTTAAAAATTAGATTGTTTTGACTCAAGCTATTCAAGTTA
GGTTTTTCTGTTAGCGAAGAGCCGTTTACTCACCGGACAATCGAATTGATTTCTATTAGT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTA
   
Sequence Name 34-K107935-0022-334-G04C-8409
GenBank Accession LN501443 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K107935-0022-334-G04C-8409
TAGAAATCATTCGATTGTCCGGTGAGTAAACGGCTCTTCGCTAACAGAAAAACCTAACTT
GAATAGCTTGAGTCAAAACAATCTAATTTTTAAATAGTTCCCAAAATTTTATCTTAAATT
ACTCGTAATTACACTAGCAATCCAAAGTTTATTTGTTTTAATAAAAACTATTTTGGGTCG
TCAAAATGGCCCATAAAGCCTAATTAAGAAGACCCAATCAATTTAAAAGCCCAAATCAGT
GTGACGTATAATATACACGTCACACGTCAATTCTCAGCGTCGTTAAAGTATGTTAACAAC
TCCGACAAGATTCGTTGCTCTAAGAATCCCATTCCGATCTTCCAATAAGATT

Last Updated on 06.12.2018 16:11