Sequences from confirmation amplicon


Confirmation sequences for line 335B03 and hit At5g64190 are shown.
Line ID 335B03
Locus At5g64190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25680515
Primers used for PCR 23vf - 8474, show primer details
   
Sequence Name 49-K117932-0022-335-B03C-23vf
GenBank Accession LN506974 [GenBank]
Sequencing primer gene specific primer, 23vf: 5'-ACTCCAATCTACGAGTCTCATGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K117932-0022-335-B03C-23vf
ATTGGCAGTAGTCACCAAAAAACCCTTTAATCTCCCACTCCATTGTTCCCCCCAATGCTC
CTTTTCCAATCTTCCACACCACACTCTCTCCATACTCGTCCCCTGCAAATATCACACCTC
CTCTCCTCGTAAATGACCGATACGCTCTTGCATACCTATCCTTAAACCAAGACCTTGGGC
TCATTATTGAAAACCGTGACGGCTTTGTCGAAAAAACCTCTCTTCCTCCAACGCTGCTAT
CATACAAGATCCAGTTTAGATTAGCTGTGTAGCCGAGCACAGATTGCTCTAATTTCCACG
GGGTCATTGTCATGGTTGAGGCTTCTCGTCCAGCAACTCTTAAGCCTAGTGAGTTTGGTG
GATCGAACGAGCCTTCAATAGATCTCTCGACTTCAAACTCTCTTCCCGGATTGTTTGATG
ATTTGCTAACCGAAACGCTAATGAAATCGGTTTGGAGAGTAGGAGTTAGTTGCAGAGAGA
TCCTTGATGGGAAATGCTTTTCGTGCTCCCCTGCTCCTCTTCTCGACATCAGTTTCTCGT
TTACAAGCTTTATTACATCGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATGTGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGC
   
Sequence Name 09-K117931-0022-335-B03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K117931-0022-335-B03C-8409
GATGAGTATGCGCTAGCGTATCCGTCATTTACAAGGATATGAAGAGTGAGATTTGCTTGG
GAAGAGAATGGAGAGAGGGAGGGGTGGAAGATTGCAAAAGGAGCATTGGGGGGAACAATG
TTGTGGGAGATTAACGGGTTTACTTGGTTGATGGCTTGGTGTCCGGAAAATCTATCTGGA
TCACCAATGAGTAGGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11