Sequences from confirmation amplicon


Confirmation sequences for line 337A05 and hit At4g02750 are shown.
Line ID 337A05
Locus At4g02750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1222435
Primers used for PCR 24hw - 8474, show primer details
   
Sequence Name 43-K112689-0022-337-A05C-24hw
GenBank Accession LN504893 [GenBank]
Sequencing primer gene specific primer, 24hw: 5'-AAGAAGAAAAAGATTGTTGAGGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K112689-0022-337-A05C-24hw
GATCATCACTGGTTATGCACAGAGCGGGAAAATCCATGAGGCGCGGCAGCTATTTGATGA
GTCTCCTGTTCAAGATGTCTTTACATGGACAGCAATGGTGTCAGGATATATACAAAACCN
GGATGGTGGAAGAAGCGAGAGAATTATTCGACAAAATGCCAGAGAGAAACGAAGTGTCAT
GGAATGCCATGCTTGCAGGATATGTGCAGGGCGAGAGAATGGAGATGGCAAAGGAATTGT
TTGATGTTATGCCTTGTAGAAATGTTAGTACATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 11-K112688-0022-337-A05C-8409
GenBank Accession LN491187 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K112688-0022-337-A05C-8409
CATTTCTACAGGCATAACATCAAACAATTCCTTTGCCATCTCCATTCTCTCGCCCTGCAC
ATATCCTGCAAGCATGGCATTCCATGACACTTCGTTTCTCTCTGGCATTTTGTCGAATAA
TTCTCTCGCTTCTTCCACCATCCGGTTTTGTATATATCCTGACACCATTGCTGTCCATGT
AAAGACATCTTGAACAGGAGACTCATCAAATAGCTGCCGCGCCTCATCGATTTTCCCGCT
CTGTGCATAACCAGTGATGATCGTATTCCATGAGACCACATCCCTCACATTCATACTATC
AAAAAACTGCCTTGCCTCAACAATTNTTATCTTCT

Last Updated on 06.12.2018 16:11