Sequences from confirmation amplicon


Confirmation sequences for line 337D04 and hit At1g75510 are shown.
Line ID 337D04
Locus At1g75510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28348772
Primers used for PCR 24nb - 8474, show primer details
   
Sequence Name 24-K114626-0022-337-D04C-24nb
GenBank Accession LN497100 [GenBank]
Sequencing primer gene specific primer, 24nb: 5'-ATCCTCGGTATTTTGTTTCCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114626-0022-337-D04C-24nb
TCTTATAGCACAATAGTGGTCCAAAGTCCAACAATATGTATGTGATCCATTTAAATTTAA
TACATTTATACATGCACCATACATCCATGATCCATGCATGCTCTACATGTGTTTATGTAT
GCAGTATGGACATACATATACCCCTATTAATCATGTCATCTATACTTTAGAAAATAAAAC
AATTCAAAATGTGGCAACAAGAAATATATGTTTTTGCGAATAACAAACGGCCCATTAAAG
AAACTGGATTCTTACAATAATATGGGCCTTGGCCTATTTGTTTTAACGCCCAAGTCGGTC
CACGTATATAGTAACGGGTCGGGTCATATGAGATGGGATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATATACGT
GGACCGACTTGGGCGTTAAAACAAATAGGCCAAGGCCCATATTATTGTAAGAATCCAGTT
   
Sequence Name 24-K114625-0022-337-D04C-8409
GenBank Accession LN497099 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114625-0022-337-D04C-8409
TTGAATCCATCTCATATGACCCGACCCGTTACTATATACGTGGACCGACTTGGGCGTTAA
AACAAATAGGCCAAGGCCCATATTATTGTAAGAATCCAGTTTCTTTAATGGGCCGTTTGT
TATTCGCAAAAACATATATTTCTTGTTGCCACATTTTGAATTGTTTTATTTTCTAAAGTA
TAGATGACATGATTAATAGGGGTATATGTATGTCCATACTGCATACATAAACACATGTAG
AGCATGCATGGATCATGGATGTATGGTGCATGTATAAATGTATTAAATTTAAATGGATCA
CATACATATTGTTTGGACTTTTGGACCACTATTGTGCTGATAAAGAGTAAAGAGAACACC
AAGGTTGTTGTTGACAAAAAAGGAAACAAAATACCGAGGA

Last Updated on 06.12.2018 16:11