Sequences from confirmation amplicon


Confirmation sequences for line 337F03 and hit At2g03260 are shown.
Line ID 337F03
Locus At2g03260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:988640
Primers used for PCR 21rq - 8474, show primer details
   
Sequence Name 42-K116218-0022-337-F03C-21rq
GenBank Accession LN504593 [GenBank]
Sequencing primer gene specific primer, 21rq: 5'-TTTAGTCGTCCTTTTCATCGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K116218-0022-337-F03C-21rq
AGCTCGGACTTCCCAACATTATTCAATGCTCATTCTCTACCCTGTTTTTATAAAGAAGAC
TTTCAAAAACCCATGATAGAAAAAACGGTGAATAAGAAACCAAAGTAACAATGTGTATAG
TAAGTAAACCTGAAGAAATTCCACATCCCACGGCGCATAATCTCAAGACTAGCCACAACC
GCAAGCGTTGTCTGCTTGTGCAAGAACTCGAATTCGAAGTTCAGTATCGTTTGCAACCAC
GCGAATCTCAACACAACGTTCAAGATCTGAAGTTTAGTTTTCAAGAATTCAGTTGTTTTT
TCTATGGTTTGGAGATTATATAATCGAAGATGTTTTAAATGCGGCTTACCATTGCGATGA
AGTATACTTTTTTCTGTGGGATGAGAAGTTTATCCCGAAGCCATCGGTTTTTAGATGTTT
TGTTCAGAAGTCCCCAATCATGAACAAAGTCCCAGTATGTACAGAAAACAGCAGCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCA
   
Sequence Name 18-K116217-0022-337-F03C-8409
GenBank Accession LN494457 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K116217-0022-337-F03C-8409
ATTTCATTGAATATATCCTGCTGCTGTTTTCTGTACATACTGGGACTTTGTTCATGATTG
GGGACTTCTGAACAAAACATCTAAAAACCGATGGCTTCGGGATAAACTTCTCATCCCACA
GAAAAAAGTATACTTCATCGCAATGGTAAGCCGCATTTAAAACATCTTCGATTATATAAT
CTCCAAACCATAGAAAAAACAACTGAATTCTTGAAAACTAAACTTCAGATCTTGAACGTT
GTGTTGAGATTCGCGTGGTTGCAAACGATACTGAACTTCGAATTCGAGTTCTTGCACAAG
CAGACAACGCTTGCGGTTGTGGCTAGTCTTGAGATTATGCGCCGTGGGATGTGGAATTTC
TTCAGGTTTACTTACTATACACATTGTTACTTTGGTTTCTTATTCACCGTTTTTTCTATC
ATGGGTTTTTGAAAGTCTTCTTTATAAAAACAGGGTAGAGAATGAGCATTTGAATAATGT
TGGGAAGTTCCGAGCTTTCAAATCAGTTCCATTACCGTTCAACTACGACGAAGACGATGA
AAAGACCGACTAA

Last Updated on 06.12.2018 16:11