Sequences from confirmation amplicon


Confirmation sequences for line 338E03 and hit At5g64500 are shown.
Line ID 338E03
Locus At5g64500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25781607
Primers used for PCR PG27 - 8474, show primer details
   
Sequence Name 42-K101745-0022-338-E03C-8409
GenBank Accession LN504411 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K101745-0022-338-E03C-8409
CGGGCTCTTTCTTTTCTACAATGATGCCTCACCAACACCAACAAACCTGAATAAGGTGAG
AAATGTAAAACGAAGATGTATAGATTTTGAAAATAAAATATGAAAAGAAATAATGGAAGA
TCTAAACACTTACATGCGACAGATTGTGATGGACCAAAAATCAAACGAAAGACCGCAGCC
AATCACAGCGAGAGTCCAGATTGACAACCCAACTCCGATAAGTCTAAACGGATTAACACT
GCATCACAGAACAGTAAGTGAGATAAATAACCTTAACGATAATGAAATATAGATTCATAT
TCAATAAAATTGATGCTTTGTACTCAGAATTTTGGAGAATCCAGTCCACACCTCTTAGCT
AAGGAAGCAAATATAGGAGAGGCAACAAGAAGCCCAACCATGAAAGCAGATGACAGAACT
CCATCTTCAAAATTGCTCAAGTTGAAATCTCCCCTGTGTAAGTTAAAACAGATTCTCATG
TTTTCACATAATATAATAGACGTCACACACTATACCTCGAATGGAGAATTTCAATACCTA
GTTCTTTAATGGATAGAGGGAAAACAAATGGAAACTTACTGTATTCCGCTGCCAGATGAA
CATGTACCACTCGAAGTGCAAGAACCACGACTCCCATTGA
   
Sequence Name 42-K101751-0022-338-E03C-PG27
GenBank Accession LN504412 [GenBank]
Sequencing primer gene specific primer, PG27: 5'-GCAAGTAATGGTATCAATGGGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K101751-0022-338-E03C-PG27
GTTCTCTGGCAGCGGAATACGTAAGTTTCCATTTGTTTTCCCTCTATCCATTAAAGAACT
AGGTATTGAAATTCTCCATTCGAGGTATAGTGTGTGACGTCTATTATATTATGTGAAAAC
ATGAGAATCTGTTTTAACTTACACAGGGGAGATTTCAACTTGAGCAATTTTGAAGATGGA
GTTCTGTCATCTGCTTTCATGGTTGGGCTTCTTGTTGCCTCTCCTATATTTGCTTCCTTA
GCTAAGAGGTGTGGACTGGATTCTCCAAAATTCTGAGTACAAAGCATCAATTTTATTGAA
TATGAATCTATATTTCATTATCGTTAAGGTTATTTATCTCACTTACTGTTCTGTGATGCA
GTGTTAATCCGTTTAGACTTATCGGAGTTGGGTTGTCAATCTGGACTCTCGCTGTGATTG
GCTGCGGTCTTTCGTTTGATTTTTGGTCCATCACAATCTGTCGCATGTAAGTGTTTAGAT
CTTCCATTATTTCTTTTCATATTTTATTTTCAAAATCTATACATCTTCGTTTTACATTTC
TCACCTTATTCAGGTTTGTTGGTGTTGGTGAGGCATCATTTGTAAGAAAAGAAAGATGCC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCANAAATGTAGATNTCCGCA
Primers used for PCR PG28 - 8474, show primer details
   
Sequence Name 43-K101745-0022-338-E03C-OriL
GenBank Accession LN504785 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K101745-0022-338-E03C-OriL
GAGCCATTTACATTGATCAGGTAGTTGCTTCTGGAATGTATCTGTTCTCTCAATCCATGG
CTTTAGATAAAGGATGGTAATTAATTGTCTATAGGAAGGAAGCTGATTATTCACATTGGC
ACTTTTGTTTTTGTTTAACCCAGAAATCAGCGTGGCTTGCTGTATTCTACATGTGCATTC
CGACAGGCTATGCTTTTGGCTACGTATATGGTGGAGTGGTGAGTTACTTTTTGTTATTCG
TTTTGTCTATGTCATGAGATTCTGATTACATGGTTCTGTTTTACCCCACTTCCAGTGACC
TCAATTCCCTCCTTCTGTTTTCTTGTAGGTGGGGAGTGTCCTTCCTTGGCGTGCAGCATT
TTGGGGAGAAGCAATACTTATGCTTCCTTTTGCTGTTCTAGGTTTTGT
   
Sequence Name 43-K101751-0022-338-E03C-PG28
GenBank Accession LN504786 [GenBank]
Sequencing primer gene specific primer, PG28: 5'-GACAAAACCTAGAACAGCAAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K101751-0022-338-E03C-PG28
CCAAAGCTGCACGCCAGGAAGGAACTCCCCACCTACAGAAAACAGAAGGAGGGAATTGAG
GTCACTGGAAGTGGGGTAAAACAGAACCATGTAATCAGAATCTCATGACATAGACAAAAC
GAATAACAAAAAGTAACTCACCACTCCACCATATACGTAGCCAAAAGCATAGCCTGTCGG
AATGCACATGTAGAATACAGCAAGCCACGCTGATTTCTGGGTTAAACAAAAACAAAAGTG
CCAATGTGAATAATCAGCTTCCTTCCTATAGACAATTAATTACCATCCTTTATCTAAAGC
CATGGATTGAGAGAACAGATACATTCCAGAAGCAACTACCTGATCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11