Sequences from confirmation amplicon


Confirmation sequences for line 339F10 and hit At1g19835 are shown.
Line ID 339F10
Locus At1g19835 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6857672
Primers used for PCR 36by - 8474, show primer details
   
Sequence Name 79-K108479-0022-339-F10C-36by
GenBank Accession LN513836 [GenBank]
Sequencing primer gene specific primer, 36by: 5'-CAGCTAATAGGCTACAAACCTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K108479-0022-339-F10C-36by
GTCCACCAGAGAGGTTTTGAATGCCAGCTGAAATCTTTTCTCGTCAAATGCTAGTAATCT
TCCTAGTATGGCTTCAATGTCTGAGGATGGAAACGAAGATGCTAGAAGCGTTGCTGGCTC
TTTGATGTCTGAACTCTCTCAATCTAACAAGGATAAAGCCAACGCGAAGATCAAGAAGAC
CGAGAGTGCTAACCAGTTGGAGCTGATGGATGATTTTCTTGAAATGGAAAAGCTAGCTTG
TCTACCAAATGGCTCAAACGCAAATGGTTCGACGGATCACTCAAGTGCTGATTCCGATGC
TGAGATTCCACCAGCGACTCAACTGAAGAAAAGAATCTCCAATGTGCTTCAGTCTCTCCC
TAAAGATGCTGCATTTGAGAAGATTTTGGCAGAGATACAATGTGCAGTTAAAGATGCAGG
TGTTAAGCTGCCTTCTAAGAGTTAATGCTGCGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATTA
   
Sequence Name 67-K108478-0022-339-F10C-8409
GenBank Accession LN511829 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K108478-0022-339-F10C-8409
ATTTACAATTGAATATATCGCAGCATTAACTCTTAGAAGGCAGCTTAACACCTGCATCTT
TAACTGCACATTGTATCTCTGCCAAAATCTTCTCAAATGCAGCATCTTTAGGGAGAGACT
GAAGCACATTGGAGATTCTTTTCTTCAGTTGAGTCGCTGGTGGAATCTCAGCATCGGAAT
CAGCACTTGAGTGATCCGTCGAACCATTTGCGTTTGAGCCATTTGGTAGACAAGCTAGCT
TTTCCATTTCAAGAAAATCATCCATCAGCTCCAACTGGTTAGCACTCTCGGTCTTCTTGA
TCTTCGCGTTGGCTTTATCCTTGTTAGATTGAGAGAGTTCAGACATCAAAGAGCCAGCAA
CGCTTCTAGCATCTTCGTTTCCATCCTCAGACATTGAAGCCATACTAGGAGGATTACTAG
CATTTTGACGAGAAAAGATTTCAGCTGGCATTTCAAAACCTCTCTTGGTTGGACTTTTAC
TCATCATTTGAGCTTCCAAGGTTTGTAGCCTATTAGCTG

Last Updated on 06.12.2018 16:11