Sequences from confirmation amplicon


Confirmation sequences for line 340B08 and hit At1g70985 are shown.
Line ID 340B08
Locus At1g70985 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26764986
Primers used for PCR BV48 - 8474, show primer details
   
Sequence Name 10-K105916-0022-340-B08C-BV48
GenBank Accession LN490657 [GenBank]
Sequencing primer gene specific primer, BV48: 5'-GAACGTATGATTCAGTTCGCTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K105916-0022-340-B08C-BV48
TTTCCTCGCTATCCTGGAAAAGCTGATCTCTGATTCCTCTCTAGACACACAATTCTGGTC
TGGTCTCGACATGGTTTTAGTTTCCTTTTAAATTGGTTAAATGAAAATTGTATTGATTTT
TATCTATACTCTGTTTCTCAAGCTGTGAGAGCTAATATGAAGGGAAACAAAGTTATATGA
AGGGAAACAAAGTTATACTTTTGGGTTAGCTTCACTAAACCAAAGAGTCGTAACATTTGT
CATTTAATAGAGGATGAACTAATCCAACTTTGTTTGCACTAAAGACTGTTTACACACTTG
AGAGACTAGAAATTACTCAACTATTTTTTTCCTCCTCGCAATTTTTATAGAAAGATTAAC
AAACATAAAAGTTTCAACCATAGAAAGTTACAATTATCCCATAAACAAAACGTTACCAAG
AAAATAACATAAGTACCGCAAGAAGATTTGCCACTGTTACGATCTTGACAGTTGATTTCA
TGGAAGATGATGACTGATCCGTCTGATGAGGAGGTTTTCTGTATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTCC
   
Sequence Name 10-K105917-0022-340-B08C-8409
GenBank Accession LN490658 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K105917-0022-340-B08C-8409
GAGCCATTTACAATTGAATACAGAAAACCTCCTCATCAGACGGATCAGTCATCATCTTCC
ATGAAATCAACTGTCAAGATCGTAACAGTGGCAAATCTTCTTGCGGTACTTATGTTATTT
TCTTGGTAACGTTTTGTTTATGGGATAATTGTAACTTTCTATGGTTGAAACTTTTATGTT
TGTTAATCTTTCTATAAAAATTGCGAGGAGGAAAAAAATAGTTGAGTAATTTCTAGTCTC
TCAAGTGTGTAAACAGTCTTTAGTGCAAACAAAGTTGGATTAGTTCATCCTCTATTAAAT
GACAAATGTTACGACTCTTTGGTTTAGTGAAGCTAACCCAAAAGTATAACTTTGTTTCCC
TTCATATAACTTTGTTTCCCTTCATATTAGCTCTCACAGCTTGAGAAACAGAGTATAGAT
AAAAATCAATACAATTTTCATTTAACCAATTTAAAAGGAAACTAAAACCATGTCGAGACC
AGACCAGAATTGTGTGTCTAGAGAGGAATCAGAGATCAGCTTTTCCAGGATAGCGAGGGA
AAGGAATAACATCTCTGATGTTATCAATTCCTGTAGCGAACT
Primers used for PCR 42wp - 8474, show primer details
   
Sequence Name 29-K118804-0022-340-B08C-42wp
GenBank Accession LN499497 [GenBank]
Sequencing primer gene specific primer, 42wp: 5'-TTTTTAATTATATCAAGCGGTGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118804-0022-340-B08C-42wp
CGTTCAATCTCAATGCCCTTGTTAGTCGTCATTATTCAATATATTCATCACTAGTCGTTT
GTCTTAACTCACATGAAACAACTTATTTTAAAAGCCACTCACTTACTGATTTCCACTCAG
ACTCAAGAAGAAAGAAAGTCTCGAAAATCTTCTCTCTTTCTCTTACACAAACATATATTT
TCTTGTTTACCATTAAACAAAATCTTTCATAAAAAATTGGCCATGAATAAACGTTACGGT
CACCTCATAAGTCTATTGTTCGTGTTTTCTCTTGTTATGTTCCCCTTCATAACAATATCA
CAGACGCCATGTCCGTACCCTTGTAACCCGTTGCCAATAGCCGGTACTGGCTCCACTCAA
CCGGCCGGTTATTATCCTCAACCGACCGGTTACTATCCTCCTCCATCGTCGGGTAATGAA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCA
   
Sequence Name 10-K118803-0022-340-B08C-oriL
GenBank Accession LN490883 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118803-0022-340-B08C-oriL
CATTTACAATTGAATATATTCATTACCCGACGATGGAGGAGGATAGTAACCGGTCGGTTG
AGGATAATAACCGGCCGGTTGAGTGGAGCCAGTACCGGCTATTGGCAACGGGTTACAAGG
GTACGGACATGGCGTCTGTGATATTGTTATGAAGGGGAACATAACAAGAGAAAACACGAA
CAATAGACTTATGAGGTGACCGTAACGTTTATTCATGGCCAATTTTTTATGAAAGATTTT
GTTTAATGGTAAACAAGAAAATATATGTTTGTGTAAGAGAAAGAGAGAAGATTTTCGAGA
CTTTCTTTCTTCTTGAGTCTGAGTGGAAATCAGTAAGTGAGTGGCTTTTAAAATAAGTTG
TTTCATGTGAGTTAAGACAAACGACTAGTGATGAATATATTGAATAATGACGACTAACAA
GGGCATTGAGATTGAACGTGTCTTGTGGCTACGATACACTTGTTGTCTTGTAAATTATGA
TACTTGGCTGTATAATTTCGTCCTTCTTTAACCAAATTGGGCCACCGCTTGATATAATTA
AAA
Primers used for PCR 12ah - 8474, show primer details
   
Sequence Name 28-K118804-0022-340-B08C-12ah
GenBank Accession LN499060 [GenBank]
Sequencing primer gene specific primer, 12ah: 5'-ATGAATAAACGTTACGGTCACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118804-0022-340-B08C-12ah
CTTCATAACATATCACAGACGCCATGTCCGTACCCTTGTAACCCGTTGCCAATAGCCGGT
ACTGGCTCCACTCAACCGGCCGGTTATTATCCTCAACCGACCGGTTACTATCCTCCTCCA
TCGTCGGGTAATGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATAATAAT
   
Sequence Name 09-K118803-0022-340-B08C-8409
GenBank Accession LN490412 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118803-0022-340-B08C-8409
CATTTACATTGAATATATTCATTACCCGACGATGGAGGAGGATAGTAACCGGTCGGTTGA
GGATAATAACCGGCCGGTTGAGTGGAGCCAGTACCGGCTATTGGCAACGGGTTACAAGGG
TACGGACATGGCGTCTGTGATATTGTTATGAAGGGGAACATAACAAGAGAAAACACGAAC
AATAGACTTATGAGGTGACCGTAACGTTTATTCAT

Last Updated on 06.12.2018 16:11