Sequences from confirmation amplicon


Confirmation sequences for line 340D09 and hit At5g36970 are shown.
Line ID 340D09
Locus At5g36970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14604884
Primers used for PCR 41qk - 8474, show primer details
   
Sequence Name 51-K115459-0022-340-D09C-41qk
GenBank Accession LN507596 [GenBank]
Sequencing primer gene specific primer, 41qk: 5'-CTTCTCCATTAACACTTGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K115459-0022-340-D09C-41qk
AACTTAAGCCGAATCAGAACATGTCCGATCACCAGAAAATTCATCCGGTGAGCGACCCCG
AAGCGCCGCCACATCCAACGGCTCCCCTTGTTCCACGTGGTTCCTCAAGATCGGAACATG
GTGATCCTACCAAGACACAGCAAGCAGCACCGTTGGATCCTCCGAGGGAGAAGAAAGGAT
CAAGAAGTTGTTGGTGCAGATGCGTGTGCTACACGCTTCTTGTCCTCTTTCTCCTTATTG
TCATCGTAGGAGCGATCGTTGGTATACTCTACCTTGTATTTAGACCAAAATTTCCGGATT
ACAATATTGACCGGTTACAGCTAACCCGGTTTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 02-K115458-0022-340-D09C-8409
GenBank Accession LN487165 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115458-0022-340-D09C-8409
GAACCGGGTTAGCTGTAACCGGTCAATATTGTAATCCGGAAATTTTGGTCTAAATACAAG
GTAGAGTATACCAACGATCGCTCCTACGATGACAATAAGGAGAAAGAGGACAAGAAGCGT
GTAGCACACGCATCTGCACCAACAACTTCTTGATCCTTTCTTCTCCCTCGGAGGATCCAA
CGGTGCTGCTTGCTGTGTCTTGGTAGGATCACCATGTTCCGATCTTGAGGAACCACGTGG
AACAAGGGGAGCCGTTGGATGTGGCGGCGCTTCGGGGTCGCTCACCGGATGAATTTTCTG
GTGATCGGACATGTTCTTGATTCGGCTTTAAGTTTGGGGTATTTTGAAGTTAATAAAGAG
AAGCAAGTGTAAATGGAGAAG

Last Updated on 06.12.2018 16:11