Sequences from confirmation amplicon


Confirmation sequences for line 340H02 and hit At3g50740 are shown.
Line ID 340H02
Locus At3g50740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18856621
Primers used for PCR BV49 - 8474, show primer details
   
Sequence Name 12-K105916-0022-340-H02C-BV49
GenBank Accession LN491579 [GenBank]
Sequencing primer gene specific primer, BV49: 5'-TGGGTCTTGAAGAGATTTCAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K105916-0022-340-H02C-BV49
CATCACACGTTGGGAAAACCGAACCGAAAGGAACAAATTCCCGGTAGAGTTGGCTGTTTG
GGTCAAGGAATGTTTCAAGTGTATCTTCAAACCGAACCGGTTCACATCCAGGCATAACCA
TAGGTTGCTTCTTGATTATGTGCTCTTCTTCCATGTCTTTGTCCAACGTTGGGAAAAACA
AAGCCACCGCGAGAAAACGTGCGTTTGAAGCGATGAAGATATAAGTCAACATGTTGAACT
CACCACCGAGCGGTATCGCGTCCAAACCAAACAAGTCTACGATCAGAGCCGTTGGTTTGT
GTTGCATCTCCTCTATCTTTGACCGGATGGTAGGAATGGTCTCACGCATCATGACCAAGA
GCTTGATCCCAAAAAAGGCTGATGGGTCGACTAAACCGGAGATATCGGGCGTTGGGAGGC
CAACGATATCAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCG
   
Sequence Name 12-K105917-0022-340-H02C-8409
GenBank Accession LN491580 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K105917-0022-340-H02C-8409
TCGGAATGAGCCATTTACAATTTGATATCGTTGGCCTCCCAACGCCCGATATCTCCGGTT
TAGTCGACCCATCAGCCTTTTTTGGGATCAAGCTCTTGGTCATGATGCGTGAGACCATTC
CTACCATCCGGTCAAAGATAGAGGAGATGCAACACAAACCAACGGCTCTGATCGTAGACT
TGTTTGGTTTGGACGCGATACCGCTCGGTGGTGAGTTCAACATGTTGACTTATATCTTCA
TCGCTTCAAACGCACGTTTTCTCGCGGTGGCTTTGTTTTTCCCAACGTTGGACAAAGACA
TGGAAGAAGAGCACATAATCAAGAAGCAACCTATGGTTATGCCTGGATGTGAACCGGTTC
GGTTTGAAGATACACTTGAAACATTCCTTGACCCAAACAGCCAACTCTACCGGGAATTTG
TTCCTTTCGGTTCGGTTTTCCCAACGTGTGATGGTATTATTGTGAATACATGGGATGATA
TGGAGCCCAAAACTTTGAAATCTCTTCAAGACCC

Last Updated on 06.12.2018 16:11