Sequences from confirmation amplicon


Confirmation sequences for line 340H06 and hit At1g55720 are shown.
Line ID 340H06
Locus At1g55720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20830214
Primers used for PCR MG34 - 8474, show primer details
   
Sequence Name 17-K032923-022-340-H06C-8409
GenBank Accession LN493750 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K032923-022-340-H06C-8409
TGTAGTCCACTACTTGACAGATAATAAGGTGAGAGCAGTTGTAGTCTCAGGTACTTATCA
TAATTAGTTTTGTTTCTTCTTGTTTAGTCTCAAGAAACGATTCTTGTTGTGGCTCAGGGG
TGGTTCTTCTTACTAAGCTTAGTAGGTATCACACCATTAGCTGAACGTCTCGGATATGCC
ACAGAGTAAGTTGTTCCTCACACTTGTGACTCTTTAATTACCATATCATCAAACACTAAC
CGTTTAGTTTTGTAACAGGCAATTGTCTTGCTACACAGGCGCAACTGGTATGACTTGGTT
TCTTCCGTTACTCTCTTTTTTTTTCTCCTAATATATTTGTGACGCGCTAGACAATTTTGA
CAGTTGGAGGTCTCCTCAATGCTACATTTGGAAACGTGATTGAGCTGATCATATCGATTA
TCGCTCTTAAAAATGGAATGATACGCGTTGTACAGTTGACGCTGCTTGGCTCGATTCTTT
CAAACATTTT
   
Sequence Name 17-K032923-022-340-H06C-MG34
GenBank Accession LN493751 [GenBank]
Sequencing primer gene specific primer, MG34: 5'-CAAAATGTTAGAAAGAATCGAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K032923-022-340-H06C-MG34
CGCGTATCATTCCATTTTAAGAGCGATAATCGATATGATCAGCTCAATCACGTTTCCAAA
TGTAGCATTGAGGAGACCTCCAACTGTCAAAATTGTCTAGCGCGTCACAAATATATTAGG
AGAAAAAAAAAGAGAGTAACGGAAGAAACCAAGTCATACCAGTTGCGCCTGTGTAGCAAG
ACAATTGCCTGTTACAAAACTAAACGGTTAGTGTTTGATGATATGGTAATTAAAGAGTCA
CAAGTGTGAGGAACAACTTACTCTGTGGCATATCCGAGACGTTCAGCTAATGGTGTGATA
CCTACTAAGCTTAGTAAGAAGAACCACCCCTGAGCCACAACAAGAATCGTTTCTTGAGAC
TAAACAAGAAGAAACAAAACTAATTATGATAAGTACCTGAGACTACAACTGCTCTCACCT
TATTATCTGTCAAGTAGTGGACTACAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11