Sequences from confirmation amplicon


Confirmation sequences for line 341B04 and hit At5g04530 are shown.
Line ID 341B04
Locus At5g04530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1293684
Primers used for PCR AY36 - 8474, show primer details
   
Sequence Name 03-K020608-022-341-B04C-8409
GenBank Accession LN487291 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K020608-022-341-B04C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAAGCCATTTTGATAACACAATTCACTTA
ATTCTCACTCACTCTCTTTCCTTCTCTACTCTCTGTTTCTTTCTTCCATGGAGCTCTTCT
CACTCTCTTCTCTTCTCCTACTCTCCACTCTCTTCGTTTTCTACATCTTCAAGTTTGTTT
TCAAAAGAAGAAACCAGAGAAACTGCTACATGCTTCACTACGAGTGCTACAAGGGCATGG
AAGAGAGAAAACTCGACACCGAGACTTGTGCTAAGGTCGTTCAACGAAACAAGAACTTGG
GTCTTGAAGAGTACAGGTTTCTCCTTCGTACTATGGCTAGTTCCGGGATAGGAGAGGAAA
CGTACGGCCCAAGAAATGTGCTTGAAGGTAGAGAAGACTCTCCTACTCTCCTTGACGCTC
ACTCCGAAATGGACGAGATCATGTTCGACACTCTCGACAAGCTTTTCCACAAGACAAAAG
GCTCAATCTCT
   
Sequence Name 04-K020608-022-341-B04C-AY36
GenBank Accession LN487734 [GenBank]
Sequencing primer gene specific primer, AY36: 5'-GAGAGATTGAGCCTTTTGTCTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K020608-022-341-B04C-AY36
GGAAAAGCTTGTCGAGAGTGTCGAACATGATCTCGTCCATTTCGGAGTGAGCGTCAAGGA
GAGTAGGAGAGTCTTCTCTACCTTCAAGCACATTTCTTGGGCCGTACGTTTCCTCTCCTA
TCCCGGAACTAGCCATAGTACGAAGGAGAAACCTGTACTCTTCAAGACCCAAGTTCTTGT
TTCGTTGAACGACCTTAGCACAAGTCTCGGTGTCGAGTTTTCTCTCTTCCATGCCCTTGT
AGCACTCGTAGTGAAGCATGTAGCAGTTTCTCTGGTTTCTTCTTTTGAAAACAAACTTGA
AGATGTAGAAAACGAAGAGAGTGGAGAGTAGGAGAAGAGAAGAGAGTGAGAAGAGCTCCA
TGGAAGAAAGAAACAGAGAGTAGAGAAGGAAAGAGAGTGAGTGAGAATTAAGTGAATTGT
GTTATCAAAATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGAT

Last Updated on 06.12.2018 16:11