Sequences from confirmation amplicon


Confirmation sequences for line 341C06 and hit At5g66690 are shown.
Line ID 341C06
Locus At5g66690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26626583
Primers used for PCR LJ03 - 8474, show primer details
   
Sequence Name 33-K027154-022-341-C06C-8409
GenBank Accession LN500854 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K027154-022-341-C06C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACCACCGTCAATGCTCAGTGACATCTCCG
CCGAGTCTCTCAACTTCTTCACTTTCCTTCTCATCGCTTCACCTTCCTTCTCAGTCATAA
CCTTCCTCACCAACGCCTCAATCTTCCACCTAGAAATATCCTCCTTTGGATCATCCAATC
TGACTGCGATTCCCAGTTCGTCGCTGAGCAACGCCGCATTCATATTCTGCTCGGCAAAAA
GTGGCCATGCGATCATCGGAACGCCGCCAACGACGCTTTCCAACGTCGAGCTCCAACCGC
AATGGGTCAAAAACCCACCAACGGCCCGATGGGACAGGATTTCAGCTTGTGGGGCCCATG
AGGGGACCACGAAACCTCTATCACT
   
Sequence Name 37-K027154-022-341-C06C-LJ03
GenBank Accession LN502484 [GenBank]
Sequencing primer gene specific primer, LJ03: 5'-CTAGTGATAGAGGTTTCGTGGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K027154-022-341-C06C-LJ03
GGCCCCACAAGCTGAAATCCTGTCCCATCGGGCCGTTGGTGGGTTTTTGACCCATTGCGG
TTGGAGCTCGACGTTGGAAAGCGTCGTTGGCGGCGTTCCGATGATCGCATGGCCACTTTT
TGCCGAGCAGAATATGAATGCGGCGTTGCTCAGCGACGAACTGGGAATCGCAGTCAGATT
GGATGATCCAAAGGAGGATATTTCTAGGTGGAAGATTGAGGCGTTGGTGAGGAAGGTTAT
GACTGAGAAGGAAGGTGAAGCGATGAGAAGGAAAGTGAAGAAGTTGAGAGACTCGGCGGA
GATGTCACTGAGCATTGACGGTGGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
Primers used for PCR LJ04 - 8474, show primer details
   
Sequence Name 21-K027344-022-341-C06C-8409
GenBank Accession LN495519 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K027344-022-341-C06C-8409
TTGTCACGTGGTGCTTAGAAATTGTTACCGTTTTCTAGCTCTTTTATTATTAGTGGTTGA
ATTATACGTGTCGTTCCTCTGTTAGTGTATAATATAATAATCGATTTACTCTTTGTAATA
TAATGATGTTTTTGATATTTTTCAACTAATTTTCCATTGTAATATTGAATAATCGGGTGT
TGTTGTAATTAATAATGAGAAACAATTTGTTACAAGAAGAGTAAATTAGTCTTCTTTCTA
TAACCGGTGTTCGAGTGCAAGACTTGATCCTACTCTCACAAAACATGATTATGGGTTTAG
CCGGTTGAGCGACGATCGACTTGGGGCAAAGATG
   
Sequence Name 22-K027344-022-341-C06C-LJ04
GenBank Accession LN495965 [GenBank]
Sequencing primer gene specific primer, LJ04: 5'-AGCATAATTTGTTTCATCTTTGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K027344-022-341-C06C-LJ04
TACGGCTACCCATATCATGTTTTGTGAGAGTAGGATCAAGTCTTGCACTCGAACACCTGG
TTATAGAAAGAAGACTAATTTACTCTTCTTGTAACAAATTGTTTCTCATTATTAATTACA
ACAACACCCGATTATTCAATATTACAATGGAAAATTAGTTGAAAAATATCAAAAACATCA
TTATATTACAAAGAGTAAATCGATTATTATATTATACACTAACAGAGGAACGACACGTAT
AATTCAACCACTAATAATAAAAGAGCTAGAAAACGGTAACAATTTCTAAGCACCACGTGA
CAAGTCCACGACACGTCATCTCCGCCGTCAATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11