Sequences from confirmation amplicon


Confirmation sequences for line 341C12 and hit At2g41190 are shown.
Line ID 341C12
Locus At2g41190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17167577
Primers used for PCR RU46 - 8474, show primer details
   
Sequence Name 17-K024451-022-341-C12C-8409
GenBank Accession LN493695 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K024451-022-341-C12C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAGTATGATCTTTGCC
ACCGAAGAATATGTCCCCAAGGTCCCGCTTACCACCCCGATGGCCACAATGATCGAACTC
AGAATCATCTGTAGTACACAACTCAGTGTTAATCTTAAGAATCCAAATACAGAAACTCAT
CATCCCATAATAATTTCAAGAAATTGATAACAGGACAACAACAAGGCTCAATTTATATAC
CTGTGTTCTTGTTGCTTTGTTCCCCATTATCTTTATGAAGCATAAAGCTGGCATTATTAT
TGCCTGAACCATACATACATTTATGTAAAACTGTTTTGAGTGTCACATTTTTTGAAAACA
ATAATGTTCTTAAGATATTAAAGAGGATGATGGAAACTAACCACTAGTATACTGAGAAGA
GATCCTATAAGAGCCATCATAAGACCTGTGTGGAAAATAAAACCA
   
Sequence Name 18-K024451-022-341-C12C-RU46
GenBank Accession LN494143 [GenBank]
Sequencing primer gene specific primer, RU46: 5'-TTTTTGCAATTGGTTTTATTTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K024451-022-341-C12C-RU46
GTCTTATGATGGCTCTTATAGGATCTCTTCTCAGTATACTAGTGGTTAGTTTCCATCATC
CTCTTTAATATCTTAAGAACATTATTGTTTTCAAAAAATGTGACACTCAAAACAGTTTTA
CATAAATGTATGTATGGTTCAGGCAATAATAATGCCAGCTTTATGCTTCATAAAGATAAT
GGGGAACAAAGCAACAAGAACACAGGTATATAAATTGAGCCTTGTTGTTGTCCTGTTATC
AATTTCTTGAAATTATTATGGGATGATGAGTTTCTGTATTTGGATTCTTAAGATTAACAC
TGAGTTGTGTACTACAGATGATTCTGAGTTCGATCATTGTGGCCATCGGGGTGGTAAGCG
GGACCTTGGGGACATATTCTTCGGTGGCAAAGATCATACTCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11