Sequences from confirmation amplicon


Confirmation sequences for line 341G12 and hit At4g38570 are shown.
Line ID 341G12
Locus At4g38570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18033147
Primers used for PCR 15sf - 8474, show primer details
   
Sequence Name 29-K116390-0022-341-G12C-15sf
GenBank Accession LN499442 [GenBank]
Sequencing primer gene specific primer, 15sf: 5'-TGATGTTGGTACCACAAGGATAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K116390-0022-341-G12C-15sf
ACACAGAGAAGGCAATGCAGTTCAAGAGAACTCTCATGTACCCTGCCAATTTTGCATGTA
ACAACACTAATCTCATATGGTTTAAAAAAAATGAAATTTTTAAAGACAGAAGAAAGGAAG
TAGCAAAAAACCGACCAACGATATTGGGAATGTAGAGGTAGACTGACAACGTCGCCGGTC
TCTGTTTAGCCATAACAGAGAGAGAGAGACTTCAGTTTGGGCAATCTGTTCCCCAACGAA
GAAAACAAGCTACATGTCCGGAACAAACAAACTTCAGTTTGGGCAATCTGTTCCCCAATG
AATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCA
   
Sequence Name 04-K116389-0022-341-G12C-8409
GenBank Accession LN488068 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K116389-0022-341-G12C-8409
ATTTACATTGATATTCATTGGGGAACAGATTGCCCAAACTGAAGTTTGTTTGTTCCGGAC
ATGTAGCTTGTTTTCTTCGTTGGGGAACAGATTGCCCAAACTGAAGTCTCTCTCTCTCTG
TTATGGCTAAACAGAGACCGGCGACGTTGTCAGTCTACCTCTACATTCCCAATATCGTTG
GTCGGTTTTTTGCTACTTCCTTTCTTCTGTCTTTAAAAATTTCATTTTTTTTAAACCATA
TGAGATTAGTGTTGTTACATGCAAAATTGGCAGGGTACATGAGAGTTCTCTTGAACTGCA
TTGCCTTCTCTGTGTGCTTCTCCAACAAGACACTCTTCTCCCTCTTGTACTTTTTCAGGT
GACTTTATCCTTGTGGACCCAACATCAA

Last Updated on 06.12.2018 16:11