Sequences from confirmation amplicon


Confirmation sequences for line 342A06 and hit At1g30920 are shown.
Line ID 342A06
Locus At1g30920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11005025
Primers used for PCR GD63 - 8474, show primer details
   
Sequence Name 78-K033710-022-342-A06C-GD63
GenBank Accession LN513634 [GenBank]
Sequencing primer gene specific primer, GD63: 5'-CCACACACTTAACTTTAGGGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K033710-022-342-A06C-GD63
GCCTAATTTACCCTTATAGTTTACCAATTTAGTAGAAGAGCAGTATGTGTTAAGACGTAG
AAACACAAAATTCTCAGACCTAACATCAAAGCAAACTAGCACATTTTTATCCATTGCATA
ATTAATTTTAGCTAGGTAATACAAAATCCCATTGATGCAAATTGTTCCATTACTATGAGG
AAGATAGCGTAAGGGAGATTGGATCTTCCTCCACCCAACTGTTTCAGCTCCTAATGTAAG
AATATGATGATCCGTTTGATGTTTATCAAAATCGAATATCGGGATTAATACCTTGAATTG
CTTGTCAATCGGATCAAACCCTAAAAATTTACCGTAAGGCACGCTCTTGAAATCCAGTAA
AGGTGGTAAGATCACATACCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 62-K033710-022-342-A06C-8409
GenBank Accession LN510680 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K033710-022-342-A06C-8409
ATTTACATTGAATATATCCTGGGTATGTGATCTTACCACCTTTACTGGATTTCAAGAGCG
TGCCTTACGGTAAATTTTTAGGGTTTGATCCGATTGACAAGCAATTCAAGGTATTAATCC
CGATATTCGATTTTGATAAACATCAAACGGATCATCATATTCTTACATTAGGAGCTGAAA
CAGTTGGGTGGAGGAAGATCCAATCTCCCTTACGCTATCTTCCTCATAGTAATGGAACAA
TTTGCATCAATGGGATTTTGTATTACCTAGCTAAAATTAATTATGCAATGGATAAAAATG
TGCTAGTTTGCTTTGATGTTAGGTCTGAGAATTTTGTGTTTCTACGTCTTAACACATACT
GCTCTTCTACTAAATTGGTAAACTATAAGGGTAAATTAGGCATGATTAATCAAGAGTATG
TTGATGATGGTGGTTTTCCCCTAAAGTTAAGTGTGTGG

Last Updated on 06.12.2018 16:11