Sequences from confirmation amplicon


Confirmation sequences for line 342E08 and hit At3g11060 are shown.
Line ID 342E08
Locus At3g11060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3466854
Primers used for PCR 21uh - 8474, show primer details
   
Sequence Name 47-K117566-0022-342-E08C-21uh
GenBank Accession LN506320 [GenBank]
Sequencing primer gene specific primer, 21uh: 5'-ATGGAGTAGTCACATTGTCTGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117566-0022-342-E08C-21uh
ATTGTTTTGAGTGATGGACTTAAGGAACCTATGGAAGACATAAAACACATGTTTTGGTGA
GCCTGTAGATCTTGTTAGTTTCTTAATATTGCTAGAAGTCAGAATCAGAGACAACGCCAC
ATCTTGCTAGAAGTTTCTTAATATTATTTTATATTTATATTTTTATTTTTGTTAGTAAGT
TTCGTTGTGAGGTGAAGATCTTTTTAGCATGTGTATAATAAATCTAAAAAGCCTTCAAAG
ACAAGAAGTCGAAAACCCTATTATTGGGACATGTCGTATATTCTTTTGATATGTTTTAGT
TCCTATAGTTTAGGAGTTTGAGCTTATCACATACAATGACTCTTGTATAAATACACATGT
TTTGTGAATCAATAAAGATTAGTCAGTTCAATACCTAGTTCTACACCTATTTCACCATCC
CTATATTAAGTCTCTGAAACCCATCACACGAGGAGAGATAAAGCCTTTTAAAAAAGAGAG
AGAGAGAGATGAGATACTTAATGGAGAAGATGAAGAGTAAAAGAGATGTGATTGTATGCA
GAGGATTATGGTATGCAATGACTCTACTCTCCATCTTTGGCCTCTCCATATCCAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 15-K117565-0022-342-E08C-8409
GenBank Accession LN493156 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117565-0022-342-E08C-8409
ATATGGAGAGGCCAAAGATGGAGAGTAGAGTCATTGCATACCATAATCCTCTGCATACAA
TCACATCTCTTTTACTCTTCATCTTCTCCATTAAGTATCTCATCTCTCTCTCTCTCTTTT
TTAAAAGGCTTTATCTCTCCTCGTGTGATGGGTTTCAGAGACTTAATATAGGGATGGTGA
AATAGGTGTAGAACTAGGTATTGAACTGACTAATCTTTATTGATTCACAAAACATGTGTA
TTTATACAAGAGTCATTGTATGTGATAAGCTCAAACTCCTAAACTATAGGAACTAAAACA
TATCAAAAGAATATACGACATGTCCCAATAATAGGGTTTTCGACTTCTTGTCTTTGAAGG
CTTTTTAGATTTATTATACACATGCTAAAAAGATCTTCACCTCACAACGAAACTTACTAA
CAAAAATAAAAATATAAATATAAAATAATATTAAGAAACTTCTAGCAAGATGTGGCGTTG
TCTCTGATTCTGACTTCTAGCAATATTAAGAAACTAACAAGATCTACAGGCTCACCAAAA
CATGTTGTTTTATGTCTTCCATAGGTTCCTTTAAGTCCATCTACTCAAAACAAATCCAAC
ACTGCTTTAGCAAAATAGTTTGCACAGACAATGTGACTTACTCCA

Last Updated on 06.12.2018 16:11