Sequences from confirmation amplicon


Confirmation sequences for line 342G08 and hit At1g61630 are shown.
Line ID 342G08
Locus At1g61630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22744347
Primers used for PCR 04ih - 8474, show primer details
   
Sequence Name 32-K112776-0022-342-G08C-04ih
GenBank Accession LN500651 [GenBank]
Sequencing primer gene specific primer, 04ih: 5'-TACTCGAAAATTGTGAAGAACGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K112776-0022-342-G08C-04ih
GTAGAACCCTAGAGGTTGATTGTTAGTCGTTTCAATAATAGACGAAGTTTCTTTGCTAGT
AACTAAATTTTATATATTTACAGTTGTTTTAAAGATTTTGAAGATTTAGGGGATTTGTGA
TCGCCTGATGACTAATCCAGAGGATATCCCTAGCAGGATCGAGGTTAGTGTACAGTTTCA
TATTAATCAATCATTCATAAATTACACACTACATAGTAATTATTATATATAGTGATATAT
GCAACTTCTGAAATAATTTTGATGATGAGTATGACTTGTGTCATACAATTGACTTATTAT
GATGAGCCATACATAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 09-K112774-0022-342-G08C-8409
GenBank Accession LN490254 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K112774-0022-342-G08C-8409
ATCCTATGTATGGCTCATCATAATAAGTCAATTGTATGACACAAGTCATACTCATCATCA
AAATTATTTCAGAAGTTGCATATATCACTATATATAATAATTACTATGTAGTGTGTAATT
TATGAATGATTGATTAATATGAAACTGTACACTAACCTCGATCCTGCTAGGGATATCCTC
TGGATTAGTCATCAGGCGATCACAAATCCCCTAAATCTTCAAAATCTTTAAAACAACTGT
AAATATATAAAATTTAGTTACTAGCAAAGAAACTTCGTCTATTATTGAAACGACTAACAA
TTCAACCTCTTAGGGTTTCTTACATATGAATAAACTCTAACTTTTTGAATCACGTTCTTC
ACATNTTTCGAGT

Last Updated on 06.12.2018 16:11