Sequences from confirmation amplicon


Confirmation sequences for line 343B06 and hit At1g32170 are shown.
Line ID 343B06
Locus At1g32170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11576365
Primers used for PCR NB31 - 8474, show primer details
   
Sequence Name 31-K101268-0022-343-B06C-8409
GenBank Accession LN500089 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101268-0022-343-B06C-8409
CCGGAATCTCTCTTCCGACCTCTATGTGTACTTCCATTTCCGTATAGATTTGTCTGAAAC
CTCCACGGCTTTCCTTTTATGTTCCCTAGAAACTCTATGTCTAACTCGTCGTGTGTTTTC
TCGAACACGTCTCCGTTTGATGTCTACAACAAAATCAAAATATAAATTCAGCTTTAGTAA
CCATTTAACATAAAATTAGAATTTAAAGATTGGTGCTCTTTTTCTTGGGAAACATAGAAC
TATTTTCTTGGGAAAATTAATGACTATTTTCTAGGCAGCCAAACAGGAAAAAATGTGACA
TGGGTTCTTCGACAAAAAGTGTGGTATTGACGATTTTCTCGGCCACAAAAAAAAAAGAGA
TTTTAAATTAGATCAAATTAGTTAGTTCATGATCTTTTGAAAACAAATAAGGAAAACTAT
CTGCAAATAATTAAATAACTAAAAGAAAACAAAAAACATTCATGCAGATGGATGAAAGAA
ACGAAAAAAAAAACATTTTAGTGGTAATGAACCAGAAAAAAAAAAAAAAAAACCAGGGGG
CGGGAAATTTCCTAAAAGGGGAAAAAAA
   
Sequence Name 31-K101274-0022-343-B06C-NB31
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NB31: 5'-GTCGTCGCCTTTTATGTAAGTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101274-0022-343-B06C-NB31
AAAAAGGTTTTTTTTTCCGTTCCTTCCCCCCCTTAAAAGGAAGGTTTTTGGTTTCCTTTA
AGTTATTAACCCTTTGGCAAAAGGTTTCCATAATTGGTTTCCAAAAAACCAGGAACAACC
AAATTGGACCAAATTAAAAACCCCTTTTTTTTTGGGGGCCAAAAAAACCCCCAAACCCCC
CTTTTTGGCCAAAAACCCCGGGCCAATTTTTTCCCGGTGGGGCGCCCAAAAAAAAACCCT
TAATTTTCCCCAAAAAAAATTTTTTTTTTTCCCCAAAAAAAAAACCCCCACCTTTAAATT
CCAATTTTTGGGTAAAGGGGTCCCAAAGCCAAAATTTATTTTGGGATTTTGGGGAAAACC
CCAAAGGGAACCGGGTCCAAAAAACCCCCCACCAGGTAAACCTAAGGTTTCCGGGGGAAC
AAAAAGGGAACCCCGGGGGGGTTCCAAAAAATCCTTCCCGGAAGGGGAATCCCCCATGGG
GGCGGGGAAAAAAAATCCCCGGAAACCCCCCGGGGACCCCCAGGGAGAAAAAGGGCCAAT
GGGGAAAAAAAAAAAAAGAATTCCCCTTTTTTTTTCCATTCCAAAAGGGGAATTCCCCCC
CCCTTTTAAAAAA

Last Updated on 06.12.2018 16:11