Sequences from confirmation amplicon


Confirmation sequences for line 343D07 and hit At5g44090 are shown.
Line ID 343D07
Locus At5g44090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17745734
Primers used for PCR CK58 - 8474, show primer details
   
Sequence Name 21-K019984-022-343-D07C-CK58
GenBank Accession LN495472 [GenBank]
Sequencing primer gene specific primer, CK58: 5'-ATTTGTTTCATAAATTGCTCACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K019984-022-343-D07C-CK58
AAATGGAATGTGGCATAGTAATTTTAATACCTGATTGTTATCTTTTTATTTATTTATGCC
ACTCGAATATAATTTCTTTTTACTTTCAATAAATTTAGTGTGCTCGATTTTATATATTGT
TTAGAACATTTGTGATAAGTAATATTGAAGTTTCTACCCTGTAGATATTGTTTGGAATTG
ATACATCTTTAAATTAGCCAAATCATGAGTAATATCATAGAAATATACAGCAGCTGTAAT
CTTTCTGTTTAGCTAGTATCTTTTAAATACACCATATTTGGTTCCTGCAGGGATGCATTT
ATCAAGTATTGGGTTGATGGACATATGTTGGCAATGGACGTAGCCTCACAGATATATAAT
ATTTTAAGGCAGCCAGGTTGCAAGTACCTTAGACAGGTCTCTTTGTTTTCTTTTCCCAGC
TCTTCACTGCTGTTTTGATTTGTTTACCAATGATTATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 22-K019984-022-343-D07C-8409
GenBank Accession LN495919 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K019984-022-343-D07C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATAATCATTGGTAAACAAATCA
AAACAGCAGTGAAGAGCTGGGAAAAGAAAACAAAGAGACCTGTCTAAGGTACTTGCAACC
TGGCTGCCTTAAAATATTATATATCTGTGAGGCTACGTCCATTGCCAACATATGTCCATC
AACCCAATACTTGATAAATGCATCCCTGCAGGAACCAAATATGGTGTATTTAAAAGATAC
TAGCTAAACAGAAAGATTACAGCTGCTGTATATTTCTATGATATTACTCATGATTTGGCT
AATTTAAAGATGTATCAATTCCAAACAATATCTACAGGGTAGAAACTTCAATATTACTTA
TCACAAATGTTCTAAACAATATATAAAATCGAGCACACTAAATTTATTGAAAGTAAAAAG
AAATTATATTCGAGTGGCATAAATAAATAAAAAGATAACAATCAGGTATTAAAATTACTA
TGCCACATTCCATTTCTTTTAAGGGTGAGCAATT

Last Updated on 06.12.2018 16:11