Sequences from confirmation amplicon


Confirmation sequences for line 343G06 and hit At3g63460 are shown.
Line ID 343G06
Locus At3g63460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23434787
Primers used for PCR BM87 - 8474, show primer details
   
Sequence Name 13-K104415-0022-343-G06C-BM87
GenBank Accession LN492025 [GenBank]
Sequencing primer gene specific primer, BM87: 5'-AAATTGGCATCTACAATATCGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K104415-0022-343-G06C-BM87
ATTGTAATTATTTTGTTCTATATGAATGAATGTAAATAATGTATGAGGCATAACAAATAA
TGAACTTTTGAATCCAGCCCTATCGACTGTTCTCCTTCATCATTTTAGGTTTCAGGTCCC
CAGTTAACATACCACTGGAAATCAAATGTTTTAGAGTTTAATCTTATACACTTTGTAGTT
GAGATTTACCTTATTAGCAAGACCTGTTGAACTGTAGATCTCCCTTCTCCCCTCCTAGAA
TGGATTAAAAAAACGTATTCAACGCTTTGGTGCAGGGTTGCAGTCGCTATGGTGTTGAAG
AGAATAATTTTGGTACTGGTAATTCCATTTACCCAATCTGAACCGTTTGTTTACCGTTGA
TTACTTCTATCATTTTTATGCTGGAAGATGAAAAGCTTCAGGACCAACATTACTTCTTAT
ATTCCTCTCTATGCAGCTCCTTTAAAAGCACCAAAATGGTATAAGCGCCCGGTTGGTGCA
TCTTTTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 29-K104416-0022-343-G06C-8409
GenBank Accession LN499272 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K104416-0022-343-G06C-8409
TTCGGAAGAGCACAACCGGGCGCTTATACCATTTTGGTGCTTTTAAAGGAGCTGCATAGA
GAGGAATATAAGAAGTAATGTTGGTCCTGAAGCTTTTCATCTTCCAGCATAAAAATGATA
GAAGTAATCAACGGTAAACAAACGGTTCAAATTGGGTAAATGGAATTACCAGTACCAAAA
TTATTCTCTTCAACACCATAGCGACTGCAACCCTGCACCAAAGCGTTGAATACGTTTTTT
TAATCCATTCTAGGAGGGGAGAAGGGAGATCTACAGTTCAACAGGTCTTGCTAATAAGGT
AAATCTCAACTACAAAGTGTATAAAATTAAACTCTAAAACATTTGATTTCCAGTGGTATG
TTAACTGGGGACCTGAAACCTAAAATGATGAAGGAGAACAGTCGATAGGGCTGGATTCAA
AAGTTCATTATTTGTTATGCCTCAAACATTATTTACATTCATTCATATAGAACAAAATAA
TTTACAATTACTGAAGGGCAAACTACTAAGGTACCTCGATATTGTAATGGCCAAT

Last Updated on 06.12.2018 16:11