Sequences from confirmation amplicon


Confirmation sequences for line 344F12 and hit At3g58760 are shown.
Line ID 344F12
Locus At3g58760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21731529
Primers used for PCR 21kh - 8474, show primer details
   
Sequence Name 73-K113549-0022-344-F12C-21kh
GenBank Accession LN512937 [GenBank]
Sequencing primer gene specific primer, 21kh: 5'-ATGGCTCCAGAGGTTTATAGGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K113549-0022-344-F12C-21kh
TGTATTCTCTTTCGCTTTATCTTACAGAGGTAAAGTAATCGAGAGAGCTATTAAGTTTTT
CTATTCATAGAGTAAAAATCCTCCGGCTTAAACAATTTATAAACAGAATGTTGTTTTGTG
GGTTAACAGAATGTTGTTTTGTGGGTTAACAAAACCTAACAGATGATAGAAGGTTGTGAA
CCATTTCATGAGATAGAAGACCGCGAAGTTCCTAAAGCATATATTGAAGATGAACGTCCA
CCATTCAATGCTCCAACAAAATCATATCCTTTCGGGTTACAAGAGTAAGTTTGTCGAAAA
AGTGTCCTGTTTTTGAAACCAACATTCGTTCATGGCTCCTCTCTGAAAGTCTTAAAATCT
CTATCTATTTGCCAGGCTAATCCAGGATTGTTGGGACAAAGAAGCATCAAAAAGACCAAC
ATTTAGAGTAATCATCTCTACTTTGGAGTTGATAAGTGATCGAATTGCACGCAAAAGGAG
ATATTCAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACGGTTTA
   
Sequence Name 08-K113548w-0022-344-F12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K113548w-0022-344-F12C-8409
GCATTTCATTGAATATATCCTGTTGATATCTCCTTTTGCGTGCAATTCGCTTTCTTATCA
ACTCCAAAGTAGAGATGATTACTCTAAATGTTGGTCTTTTTGATGCTTCTTTGTCCCAAC
AATCCTGGATTAGCCTGGCAAATAGATAGAGATTTTAAGACTTTCAGAGAGGAGCCATGA
ACGAATGTTGGTTTCAAAAACAGGACACTTTTTCGACAAACTTACTCTTGTAACCCGAAA
GGATATGATTTTGTTGGAGCATTGAATGGTGGACGTTCATCTTCAATATATGCTTTAGGA
ACTTCGCGGTCTTCTATCTCATGAAATGGTTCACAACCTTCTATCATCTGTTAGGTTTTG
TTAACCCACAAAACAACATTCTGTTAACCCACAAAACAACATTCTGTTTATAAATTGTTT
AAGCCGGAGGATTTTTACTCTATGAATAGAAAAACTTAATAGCTCTCTCGATTACTTTAC
CTCTTGTAAGATTAAAGCGAAAGAGAATACATCTACTTTTGTATCATACTCTTCATTCCT
ATAAACCTTGGGAGCCA

Last Updated on 06.12.2018 16:11