Sequences from confirmation amplicon


Confirmation sequences for line 345B03 and hit At5g60580 are shown.
Line ID 345B03
Locus At5g60580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24355258
Primers used for PCR 24lm - 8474, show primer details
   
Sequence Name 29-K114053-0022-345-B03C-24lm
GenBank Accession LN499382 [GenBank]
Sequencing primer gene specific primer, 24lm: 5'-AAGATTCGGTTGTTATCAAAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114053-0022-345-B03C-24lm
CACTTTGCTTTGACTTCGAGTTCTCCAGGTCCTACTCCTACTTCTTCTTCTGTTCTTCCT
CGTGGCAAATCGTCCCTGAAGAATCTATTGCCAAAAGCTGGGTGTAAACCAAAAACCTCT
AACACTGATATCGAGAAGGGACAAGGGAATGCCTGTTCTCCTCCTGCATCGCAGGAGAAG
GCTTCGATATCGAGGTCATTATCACTTTCAAAGCTATTTACACCTAGGATCAAGAGAACT
TCGTCCCTACCTGTGACTCCTGTTATTCTTTCAAACTCAGAGTCAGCTCACGGAGGAACC
AGTGTTGCTCCTCAAACTCCAAACGTAAGCTTTCTCTTGTATCATTTCAGAGAACCTTAT
GATTGTTTAGGTACTTATACTTGGCATTGCAGAGAAAAGGGAGTGTACACATAGCTCGCT
CTACATGGCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCG
   
Sequence Name 13-K114052-0022-345-B03C-8409
GenBank Accession LN492127 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K114052-0022-345-B03C-8409
ATTTCATTGAATAAGCCATGTAGAGCGAGCTATGTGTACACTCCCTTTTCTCTGCAATGC
CAAGTATAAGTACCTAAACAATCATAAGGTTCTCTGAAATGATACAAGAGAAAGCTTACG
TTTGGAGTTTGAGGAGCAACACTGGTTCCTCCGTGAGCTGACTCTGAGTTTGAAAGAATA
ACAGGAGTCACAGGTAGGGACGAAGTTCTCTTGATCCTAGGTGTAAATAGCTTTGAAAGT
GATAATGACCTCGATATCGAAGCCTTCTCCTGCGATGCAGGAGGAGAACAGGCATTCCCT
TGTCCCTTCTCGATATCAGTGTTAGAGGTTTTTGGTTTACACCCAGCTTTTGGCAATAGA
TTCTTCAGGGACGATTTGCCACGAGGAAGAACAGAAGAAGAAGTAGGAGTAGGACCTGGA
GAACTCGAAGTCAAAGCAAAGTTGACTCTTCTTGGAGTTGGACTAGGCGTTGGAGGCATT
TTGAT

Last Updated on 06.12.2018 16:11