Sequences from confirmation amplicon


Confirmation sequences for line 345B09 and hit At5g66310 are shown.
Line ID 345B09
Locus At5g66310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26489384
Primers used for PCR 09ie - 8474, show primer details
   
Sequence Name 27-K112776-0022-345-B09C-09ie
GenBank Accession LN498464 [GenBank]
Sequencing primer gene specific primer, 09ie: 5'-TTCAAAGATATGTACCACTGTCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K112776-0022-345-B09C-09ie
GAGCTATGCTTTCTACTGTCTGTAAGAAGGTAAGAGTGCAAAGATATAAGTTTTAAAACA
TGATGAAGAAGGCAGTGTTGAACCATATATAGAAATCAACAAGATGCCTTACCAATCTCA
AAATCTGATGAGACCGGGAGCTAACTTCATTTAGAGCTGTCTCACCAATTTGCCTTTGTG
CTGCAAATGGTTAAAACACTTGTCAATGTTGTTGCAAAACATCACTAGTATACCATTATT
GATTATCTATACCTTTACACTATGAGCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 04-K112774-0022-345-B09C-8409
GenBank Accession LN487989 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K112774-0022-345-B09C-8409
ATTTCATTGAATTATCCTGCTCATAGTGTAAAGGTATAGATAATCAATAATGGTATACTA
GTGATGTTTTGCAACAACATTGACAAGTGTTTTAACCATTTGCAGCACAAAGGCAAATTG
GTGAGACAGCTCTAAATGAAGTTAGCTCCCGGTCTCATCAGATTTTGAGATTGGTAAGGC
ATCTTGTTGATTTCTATATATGGTTCAACACTGCCTTCTTCATCATGTTTTAAAACTTAT
ATCTTTGCACTCTTACCTTCTTACAGACAGTAGAAAGCATAGCTCGTGAATTTTCTACCA
ATGATAAGTTTAGTACTCTAACAGCGACAGTGGTACATATC

Last Updated on 06.12.2018 16:11