Sequences from confirmation amplicon


Confirmation sequences for line 346F04 and hit At2g37160 are shown.
Line ID 346F04
Locus At2g37160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15611022
Primers used for PCR 18et - 8474, show primer details
   
Sequence Name 56-K110926-0022-346-F04C-18et
GenBank Accession LN509133 [GenBank]
Sequencing primer gene specific primer, 18et: 5'-AAATGCAATAGGGAAGATCATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K110926-0022-346-F04C-18et
AGCACACACAATAGAGCACCATAATAACTTTTTACACCACACACTAGCTTTTGGGTTGAA
AAATCAAAAATTCGAAGATAACCTGTACATGGTCAAAAGAGAAAATGAATTCTCAACTGA
AAGATATATATAGAGAGAGGAAGAATTGGAGCCGGCAATAAAACAAAAACCCATCATACC
ATCTCTTCCAACAGTTGCCAAGTACGCTCCATCATTTGAAAATGCAATGCTATTAATCGC
ACCTTGCCCAATATGCCATCTCGCTACTGGCATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 40-K110925-0022-346-F04C-8409
GenBank Accession LN503740 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K110925-0022-346-F04C-8409
AGCGAGATGGCATATTGGGCAAGGTCCGTTTAATAGCATTGCATTTTCAATGATGGAGCG
TACTTGGCAACTGTTGGAAGAGATGGTATGATGGGTTTTTGTTTTATTGCCGGCTCCAAT
TCTTCCTCTCTCTATATATATCTTTCAGTTGAGAATTCATTTTCTCTTTTGACCATGTAC
AGGTTATCTTCGAATTTTTGATTTTTCAACCCAAAAGCTAGTGTGTGGTGTAAAAAGTTA
TTATGGTGCTCTATTGTGTTGTGCTTGGAGGTAACTGGATCTAAATCCTTTGACAAATGA
TCTTCCCTATAGCATT

Last Updated on 06.12.2018 16:11