Sequences from confirmation amplicon


Confirmation sequences for line 347C04 and hit At2g35480 are shown.
Line ID 347C04
Locus At2g35480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14910828
Primers used for PCR BV60 - 8474, show primer details
   
Sequence Name 35-K106149-0022-347-C04C-BV60
GenBank Accession LN501840 [GenBank]
Sequencing primer gene specific primer, BV60: 5'-AAACTAGAAACGGAGGATCTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K106149-0022-347-C04C-BV60
TGATTCTGGCGGCTCTTTCAAGGAAAATTGTAGATTGTATTCAAATACGTTGAGGATGAA
AGAAGGTGACAAAGAGGAAGACGAAGAAGAAAGTTGTAGAAAGTAATGGTGTTGTGGGAG
TTAGCTCTAGGAACAGCTTACTTCTTGGGGATTAGGCGGACTTACCGGCTCGCATTGAAG
ACCCAGCGTCGGCTTGTTAGCCCTAAGCATCCAAGGATTCGAGATTTTATGCATCGGTGA
AGTTTCTTTCATCTTTACGCTTTTCTTTTTGTGGATTTTGCGATTTGGTTGTTTCTGATT
TAGGTTTGACTGGAAATACGTTTCTTTGTATATGAATTGGCCTCTTCAATTGATGAAACT
GCTTTGTTATTGATGTATCGTTCTGATTGTTTATTGTGTTTAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTA
   
Sequence Name 51-K106150-0022-347-C04C-8409
GenBank Accession LN507508 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K106150-0022-347-C04C-8409
TCGGAATGAGCCTTTACAATTGAATTATCCTAAACACAATAAACAATCAGAACGATACAT
CAATAACAAAGCAGTTTCATCAATTGAAGAGGCCAATTCATATACAAAGAAACGTATTTC
CAGTCAAACCTAAATCAGAAACAACCAAATCGCAAAATCCACAAAAAGAAAAGCGTAAAG
ATGAAAGAAACTTCACCGATGCATAAAATCTCGAATCCTTGGATGCTTAGGGCTAACAAG
CCGACGCTGGGTCTTCAATGCGAGCCGGTAAGTCCGCCTAATCCCCAAGAAGTAAGCTGT
TCCTAGAGCTAACTCCCACAACACCATTACTTTCTACAACTTTCTTCTTCGTCTTCCTCT
TTGTCACCTTCTTTCTTCCTCAACGTATTTGAATACAATCTACAATTTTCCTTTGAAAGA
GCCGCCAGAAATTTCAATCTCACTCCCATCGGAATCACAGATCCTCCGTTTCTAGTT

Last Updated on 06.12.2018 16:11