Sequences from confirmation amplicon


Confirmation sequences for line 348D02 and hit At5g61940 are shown.
Line ID 348D02
Locus At5g61940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24872472
Primers used for PCR 22qk - 8474, show primer details
   
Sequence Name 59-K115459-0022-348-D02C-22qk
GenBank Accession LN510074 [GenBank]
Sequencing primer gene specific primer, 22qk: 5'-TGAAGGAAAGACTCTAACTCGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K115459-0022-348-D02C-22qk
GACAGAAGATACCGAGCCGGTTAAGTGGTTGTATCTATTGATGATGTTAAGCGATGTCGT
TTGAGGTAATCGCTATAAGACGTTTAGCTCAAGCTCTGCATTTTCTTTCAATCTTTTTTT
TCTCGCTCTCTATGTTTTCTATCTTGTGTAACAACACTCTTAAGACTCAAGTGATAGTGA
ATTAAGAGGTACCGTAGTTATACTTGGATCACATCGATCTCATCAAAAAAAAAACCAAAG
CAAAGATAAAAAAGTGTCTTCGGGTAAATGGAAACATCAATGCTAAACTTCTCCACATTG
TCACTTGTTAGGCCATCGATCAGGCAGCTTCTTAAAGCAAAATCTCTGGCCAAACCTTCC
TCTCTCCACAAAATCTGACCACATTCTTCCAGTCACCAACATCCTATGCCATCACCACAC
ATATATAATTCATTCAGGTCTACCTAATCTAAATTCACTATACAATTCTAAGGTAAACAA
AAGGTAACTGTTTCAACTTACCTCTCCGGCTATACACTCACGTCTAAAATTGACCCACCG
GTTTTCTTCATAAGCTAACCATATGGATCATTTGTAAATGGCTTCATGTCCGGAAAATCT
ACATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTATTTACCAAT
TTTTTTTCAATTCAAAAATGTAAATTTC
   
Sequence Name 10-K115458-0022-348-D02C-8409
GenBank Accession LN490792 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115458-0022-348-D02C-8409
GCCATTTCATTGATCCATATGCTTAGCTTATGAAGAAAACCGGTGGGTCAATCTCAGACG
TGAGTGTATAGCAGGAGAGGTAAGTCGAAACAGTTACCTTTTGTCTACCTCAGAATTGTA
TAGTGAATTTAGATAAGGTAGACATGAATGATTGATATATGTGTGGTGATGGCATAGGAT
GTTGGTGACTGGAAGAATGTGGTCAGATTCTGTGGAGAGAGGAAGGTTCGGCCAGAGATT
CTGCTTTACGAAGCTGCCTGATCGATGGCCTAACAAGTGACAATGTGGAGAAGTCTAGCA
TTGATGTTTCCATCTACCAGAAGACACTTTTTTATCTTTGCTTTGGTTTTTTTTTTGATG
AAATCGATGTGATCCAAGTATAACTACGGTACCTCTTAATTCACTATCACTTGAGTCTTA
AAAGTGTTGTTCCCCAAGATAGAAAACTTAGAGAGCGAGAAAAAAAAGATTGAAAAAAAA
TGCAAAGCTTGAGCTAAACGTCTTATAGCGATTACCTCAAACGACTTCGCTAAACATCAT
CAATAGATACAACCACTTAACCGGCTCGGTTATCTTTCTGGTTCGTTTAAATCCCCAAAT
ACTCCAAGCGAGTTAAAGTCTTTTCCTT

Last Updated on 06.12.2018 16:11