Sequences from confirmation amplicon


Confirmation sequences for line 348E09 and hit At2g36010 are shown.
Line ID 348E09
Locus At2g36010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15122079
Primers used for PCR MS76 - 8474, show primer details
   
Sequence Name 07-K024135-022-348-E09C-MS76
GenBank Accession LN489088 [GenBank]
Sequencing primer gene specific primer, MS76: 5'-TTCCAGGTCTGTCTTTCCTATTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K024135-022-348-E09C-MS76
TATGTTAATCCTAGGAATTTGATCAATTGATTGTATGTATGTCGATCCCAAGACTTTCTT
GTTCACTTATATCTTAACTCTCTCTTTGCTGTTTCTGCAGAACCAGACTCTGATAGCCGT
CAAAGCTCCTCATGGCACAACTTTGGAAGTGCCTGATCCAGATGAAGTTAGTCTTTTTCA
GCGACCTTGGATGACATCTGCCATTCTTTGTTAGATTCCAAAACGTTATTATAATGTTTC
TTCTTTACTTGACAGGCGGCTGACCACCCACAAAGGAGATACAGGATCATTCTTAGAAGT
ACAATGGGACCTATTGACGTATACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 08-K024135-022-348-E09C-8409
GenBank Accession LN489552 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K024135-022-348-E09C-8409
GTAGATTTCCCGGACATGAAGCCATGTATACGTCAATAGGTCCCATTGTACTTCTAAGAA
TGATCCTGTATCTCCTTTGTGGGTGGTCAGCCGCCTGTCAAGTAAAGAAGAAACATTATA
ATAACGTTTTGGAATCTAACAAAGAATGGCAGATGTCATCCAAGGTCGCTGAAAAAGACT
AACTTCATCTGGATCAGGCACTTCCAAAGTTGTGCCATGAGGAGCTTTGACGGCTATCAG
AGTCTGGTTCTGCAGAAACAGCAAAGAGAGAGTTAAGATATAAGTGAACAAGAAAGTCTT
GGGATCGACATACATACAATCAATTGATCAAATTCCTAGGATTAAACATATGAAATAGGA
AAGACAGACCCTG

Last Updated on 06.12.2018 16:11