Sequences from confirmation amplicon


Confirmation sequences for line 349D12 and hit At1g48830 are shown.
Line ID 349D12
Locus At1g48830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18059956
Primers used for PCR 34ns - 8474, show primer details
   
Sequence Name 51-K114626-0022-349-D12C-34ns
GenBank Accession LN507585 [GenBank]
Sequencing primer gene specific primer, 34ns: 5'-CCTTTTCATGTTCCTATTGGTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K114626-0022-349-D12C-34ns
TGCTACTTTAGTTTTCTAAGAATGAAGTTATGTTCCATTGGGTTATATTGAGAATGTAAA
TAAGCTTTCTGTGTTTCAATTACCGGTTTTAGAGTCACTGTGCAATTGGTTAGCTTCTTA
GTGTTATCTTTGTCTCTCTTCCCTGTGCTTAGGATGTGATCCTCATTGCTACAAGGAGGA
TTGTGAGGCCGCCAAAGAAGGGTTCAGCGGCAAAGAGACCACGCAACCGTACTCTGACCT
CAGTCCATGAAGCTATTCTTGATGATGTGGTCTTACCTGCAGAGATTGTTGGGAAGCGCA
CAAGGTACAGACTCGACGGTACCAAAATCATGAAGGTAAGTCAAAATTCGAAGATTATTA
CCATACAGGAAGATGTAATTTTTTGTTTATCTAATAAATGGGATTTGTGTGGTTGAAGGT
CTTCTTGGACCCAAAGAAGAAAAAGAAAGAGTAATTACCAATTGATTTGTGTGTGATTGG
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCC
   
Sequence Name 51-K114625-0022-349-D12C-8409
GenBank Accession LN507584 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K114625-0022-349-D12C-8409
CCGGAAGAAGCCTTTACATTGAAACCAATCACACACAAATCAATTGGTAATTACTCTTTC
TTTTTCTTCTTTGGGTCCAAGAAGACCTTCAACCACACAAATCCCATTTATTAGATAAAC
AAAAAATTACATCTTCCTGTATGGTAATAATCTTCGAATTTTGACTTACCTTCATGATTT
TGGTACCGTCGAGTCTGTACCTTGTGCGCTTCCCAACAATCTCTGCAGGTAAGACCACAT
CATCAAGAATAGCTTCATGGACTGAGGTCAGAGTACGGTTGCGTGGTCTCTTTGCCGCTG
AACCCTTCTTTGGCGGCCTCACAATCCTCCTTGTAGCAATGAGGATCACATCCTAAGCAC
AGGGAAGAGAGACAAAGATAACACTAAGAAGCTAACCAATTGCACAGTGACTCTAAAACC
GGTAATTGAAACACAGAAAGCTTATTTACATTCTCAATATAACCCAATGGAACATAACTT
CATTCTTAAGAAAACTAAAGTAGCATCAATCCATCAATCAAACCAATGCAAGTCGAGAAA
CTTAACAGAACACCAATAGG

Last Updated on 06.12.2018 16:11