Sequences from confirmation amplicon


Confirmation sequences for line 349E11 and hit At3g52060 are shown.
Line ID 349E11
Locus At3g52060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19311067
Primers used for PCR 14ve - 8474, show primer details
   
Sequence Name 49-K118051-0022-349-E11C-14ve
GenBank Accession LN506977 [GenBank]
Sequencing primer gene specific primer, 14ve: 5'-TTTTCCCATATCTCTACCTTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118051-0022-349-E11C-14ve
AGCATTTTCACCACACACACAGAAGTTCATCTTTATTTAGCCACTCTTCTTCGAGCGCCA
ACACAAGCTTCTTCCTAAACCTTCCTTATGATGATATCTGAAACCTGAACACAATAACAA
GAAGATAAGCAAATTGAAACTAAACTCTCAAACTGTATTCATATTTACACATTGAGATTT
CAGTGTTATACCTAATTTACTTTCACCGAATTCAATCCCTAAAGATCACAGCATCTGCAA
TCTCCATAAGCGGTTGCAATGACTCCGGCGTGAATTTTCTGGCGAAGAAATATACAACAC
GTGAAATGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGC
   
Sequence Name 13-K118050-0022-349-E11C-8409
GenBank Accession LN492240 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118050-0022-349-E11C-8409
TACTCATTCACGTGTTGATATTTCTTCGCCAGAAAATTCACGCCGGAGTCATTGCAACCG
CTTATGGAGATTGCAGATGCTGTGATCTTTAGGGATTGAATTCGGTGAAAGTAAATTAGG
TATAACACTGAAATCTCAATGTGTAAATATGAATACAGTTTGAGAGTTTAGTTTCAATTT
GCTTATCTTCTTGTTATTGTGTTCAGGTTTCAGATATCATCATAAGGAAGGTTTAGGAAG
AAGCTTGTGTTGGCGCTCGAAGAAGAGTGGCTAAATAAAGATGAACTTCTGTGTGTGTGT
GTGAAAATTGCTGTGTTTTTTAAGCTTTTGTGTGTCTCTAGGGAAGGTAGAAANTTGGGA
AAA

Last Updated on 06.12.2018 16:11