Sequences from confirmation amplicon


Confirmation sequences for line 349F05 and hit At1g64430 are shown.
Line ID 349F05
Locus At1g64430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23936782
Primers used for PCR 25qi - 8474, show primer details
   
Sequence Name 18-K115435-0022-349-F05C-25qi
GenBank Accession LN494435 [GenBank]
Sequencing primer gene specific primer, 25qi: 5'-GAAAGAGAGAGCGAGAGAATCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115435-0022-349-F05C-25qi
TATACTAACCTTCTTGTTTGGGAAGGAAAAAAAGAGGAGTGGAGAAGAGAAGCCACNGTG
GCTTTTTTGTTTATACCTTATTAATTAATACTTCAATTAAGTAATTAATGTCACAATTCT
CTGGAGAAAAGTGTCACTTTGTGTGTTTAGCAACTACTAATAATTTAACTAACAAATGCT
TGCATTTAAGAAAAAAAGATTACAAATGGACAAGAAACAAGAAATTAATGATTTCGGTGA
TTATTAAACTTAAACAAACAAATCTACATGTAAATGGCTCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTT
ATTATA
   
Sequence Name 02-K115434-0022-349-F05C-8409
GenBank Accession LN487164 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115434-0022-349-F05C-8409
CATTTAATGTAGATTTGTTTGTTTAAGTTTAATAATCACCGAAATCATTAATTTCTTGTT
TCTTGTCCATTTGTAATCTTTTTTTCTTAAATGCAAGCATTTGTTAGTTAAATTATTAGT
AGTTGCTAAACACACAAAGTGACACTTTTCTCCAGAGAATTGTGACATTAATTACTTAAT
TGAAGTATTAATTAATAAGGTATAAACAAAAAAGCCACGTGGCTTCTCTTCTCCACTCCT
CTTTTTATTCCTTCCCAAACAAGAAGGTTAGTTATTATTATTTCCAGAGAAGCTTCTCTT
TTGATTCTCTCCTCCTCTCTTTCA

Last Updated on 06.12.2018 16:11