Sequences from confirmation amplicon


Confirmation sequences for line 349H05 and hit At1g57943 are shown.
Line ID 349H05
Locus At1g57943 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21438431
Primers used for PCR AC35 - 8474, show primer details
   
Sequence Name 57-K027911-022-349-H05C-8409
GenBank Accession LN509304 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K027911-022-349-H05C-8409
CTTCTTCCTTACTTATCTCTTGGTGTTCTTGTTGCTGCTCAAGCCCATATTACGCACTCG
GGAAATTAGTCTCAAACTATGGCATCTTCTCATTGATTTCCACAACACAGTTGATATTTA
CCGNCTGTTTTAACAGCCATCATTAACCGTTTCAAGTTTACTAGATGGATCATCATATCG
ATATTACTCACTATTGTGATTTACGTTCTCGGTACTCCTGACTTTGGAGGACAGCCTCAT
GACGGCGAAGAATTCGGATACAACATCCAAGCTTGGTTAGCTTTCTCTGCTA
   
Sequence Name 58-K027911-022-349-H05C-AC35
GenBank Accession LN509595 [GenBank]
Sequencing primer gene specific primer, AC35: 5'-TGAGAAAGCAATTGTAGCAGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K027911-022-349-H05C-AC35
CGAATTCTTCGCCGTCATGAGGCTGTCCTCCAAGTCAGGAGTACCGAGAACGTAAATCAC
AATAGTGAGTAATATCGATATGATGATCCATCTAGTAAACTTGAAACGGTTAATGATGGC
TGTTAAAACAGCGGTAAATATCAACTGTGTTGTGGAAATCAATGAGAAGATGCCATAGTT
TGAGACTAATTTCCCGAGTGCGAACAATTTGCTGTGAGCAGCAACAAGAACACCAAGAGA
TAAGTAAAGGAAGAAGAGACGCAAGGAGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTACT

Last Updated on 06.12.2018 16:11