Sequences from confirmation amplicon


Confirmation sequences for line 350A02 and hit At4g00520 are shown.
Line ID 350A02
Locus At4g00520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:231087
Primers used for PCR BV65 - 8474, show primer details
   
Sequence Name 36-K106149-0022-350-A02C-BV65
GenBank Accession LN502231 [GenBank]
Sequencing primer gene specific primer, BV65: 5'-TTCTTTTGTCTTAGCGAAGTGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K106149-0022-350-A02C-BV65
TAGTCAACGACTTATTCTATATATACACGCAGGCGCACACATAATTACATACCTGCTCTA
TCGTCTATTATCTTGATTTTGCCATCTTCGAAAACAATGCCTCGAGTAAAAGTTTCTTAC
TTTTCTTATGTCCCTTTGCAGGATGTGGTTCCACCGACCTGTAAGAGCAGATGATTGGTT
GTTGTTTGTGGTAAGAAAAGCTCATGTTTTTCTTCTTCTTTTTTTCTGAAGAGTAGGAGA
AGTTTTCTTAAGAAGGAAAAGTTATTACTAGTAGAAAACCAAATGTTTTGTTTAGGTCAA
CTGAAACTCAAATTGTCTAAAATACTTCGCGCAACTTTGAGTTGTTTTGGAGAACACTAG
CAACTATAGATTAGAACAACTTGCTTGTTTAGCTGATATTCGTTGAAGTGTATAGATCAA
CCACTGCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 52-K106150-0022-350-A02C-8409
GenBank Accession LN507824 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K106150-0022-350-A02C-8409
TCGGAATGAGCCATTTACATTGAATATATCCGCAGTGGTTGATCTATACACTTCAACGAA
TATCAGCTAAACAAGCAAGTTGTTCTAATCTATAGTTGCTAGTGTTCTCCAAAACAACTC
AAAGTTGCGCGAAGTATTTTAGACAATTTGAGTTTCAGTTGACCTAAACAAAACATTTGG
TTTTCTACTAGTAATAACTTTTCCTTCTTAAGAAAACTTCTCCTACTCTTCAGAAAAAAA
GAAGAAGAAAAACATGAGCTTTTCTTACCACAAACAACAACCAATCATCTGCTCTTACAG
GTCGGTGGAACCACATCCTGCAAAGGGACATAAGAAAAGTAAGAAACTTTTACTCGAGGC
ATTGTTTTCGAAGATGGCAAAATCAAGATAATAGACGATAGAGCAGGTATGTAATTATGT
GTGCGCCTGCGTGTATATATATGAATTAAGTCGTTTGTACTTATGTAGGGAGAATGATAC
AAACTCACACTTCGCTAAGACAAAAGA

Last Updated on 06.12.2018 16:11