Sequences from confirmation amplicon


Confirmation sequences for line 350A04 and hit At1g17710 are shown.
Line ID 350A04
Locus At1g17710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6091523
Primers used for PCR 02on - 8474, show primer details
   
Sequence Name 69-K114864-0022-350-A04C-02on
GenBank Accession LN512259 [GenBank]
Sequencing primer gene specific primer, 02on: 5'-CTCTAGCCTTTTCACCTTCTCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K114864-0022-350-A04C-02on
CAATTCATAATCNACAGAAATCGATAAAACAGTACCTATAAAACATTGTATCTGATAGAA
TGGCTAAGAATAACAACATCGTGATCGTCTTCGATTTTGACAAGACAATCATCGATGTAG
ACAGCGATAATTGGGTCGTGGATGAACTTGGTTTCACTGATTTGTTCAACCAGCTTCTCC
CAACAATGCCTTGGAACTCTCTCATGGTTTGTTTTGTTCATCCATCTCTAACTCTTCTCT
GTTTCGGTTTCTCTAGGGCTTTGTTACGTTGGTCTATATATGTTCATCAGTCATAACACT
AAATCCTTATTTGGTTTTATTTCAAAACAGAATCGGATGATGAAGGAGCTTCATGATCAT
GGTAAAACCATTGAAGAAATCAAACAAGTCCTTAGGAGAATCCCAATTCATCCACGTGTC
ATCCCAGCCATCAAATCTGCTCATGCTTTAGGGTAAAAATCCATGTTAAATTCCTCATTC
TTCGTTTGATAACACGGTACTGAATCATACGACATAATATGGGATCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 29-K114863-0022-350-A04C-8409
GenBank Accession LN499410 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114863-0022-350-A04C-8409
TCCCATATTAGNTCGTATGATTCAGTACCGNGNTTATCAAACGAAGAATGAGGAATTNTA
ACATGGATTTTTACCCTAAAGCATGAGCAGATTTGATGGCTGGGATGACACGTGGATGAA
TTGGGATTCTCCTAAGGACTTGTTTGATTTCTTCAATGGTTTTACCATGATCATGAAGCT
CCTTCATCATCCGATTCTGTTTTGAAATAAAACCAAATAAGGATTTAGTGTTATGACTGA
TGAACATATATAGACCAACGTAACAAAGCCCTAGAGAAACCGAAACAGAGAAGAGTTAGA
GATGGATGAACAAAACAAACCATGAGAGAGTTCCAAGGCATTGTTGGGAGAAGCTGGTTG
AACAAATCAGTGAAACCAAGTTCATCCACGACCCAATTATCGCTGTCTACATCGATGATT
GTCTTGTCAAAATCGAAGACGATCACGATGTTGTTATTCTTAGCCATTCTATCAGATACA
ATGTTTTATAGGTACTGTTTTATCGATTTCTGTATGATTATGGAATTTGGAGAAGAAGAA
TGTGATATATATACGAGTGAGAGAAGGTGAAAGGCTAGA

Last Updated on 06.12.2018 16:11