Sequences from confirmation amplicon


Confirmation sequences for line 350D01 and hit At3g15030 are shown.
Line ID 350D01
Locus At3g15030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5063198
Primers used for PCR UF33 - 8474, show primer details
   
Sequence Name 30-K030221-022-350-D01C-8409
GenBank Accession LN499599 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K030221-022-350-D01C-8409
TCGGATGAGCATTTCATTGAATTATTTGTTGTGCTCCGCCGCCACCGCCGCGTGTTCCAA
GCCACTAGTCTCTGAATCCTTCCGAATTCCGACGACTGCTGCTCCCAACTCGATGTGTCA
AACCCTAACGGATTGCTCTGTCCGTAGAACAGAGTAGGCTCGGAGGCGGAAGCAGAGGTG
TGGTGGTGATGTTGGTGGTGCAGAAGCGACGGTGGACCATCCGGGAACGATTGCAGCGAG
AGACGGAGATCTTGGTTTTGGCTATTAGTTCGAGAAAGCAAATCCGGCGGATGCTGATGA
TGATAGTTGTGCATAAGGTTATGATTCGAAGGAGCCTCCGTTGAAGAGCCAATCACCGGA
AAAAACGACTTTATAGTGTCAGCTATCGAATCTGAATCCATTGACGGCGGGAGAAAACTC
GACTCNGTTGTTA
   
Sequence Name 31-K030221-022-350-D01C-UF33
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, UF33: 5'-GTAACAACGAGTCGAGTTTTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K030221-022-350-D01C-UF33
TTCCGATTTTTTTTCAATTCAAAAATGTACAGGTCCTCCGGATTATTATAAAAGGAAAGT
ACGTTTTGATACAACGGCACATTACGATCCGTCGCATTTATAGGCGAAAACAACAAACAA
ATGATTCTAATTCGGAAATCTTTCTTTGGACGTGGCTACATACACGTCCAAATGGGA

Last Updated on 06.12.2018 16:11