Sequences from confirmation amplicon


Confirmation sequences for line 350E03 and hit At1g11170 are shown.
Line ID 350E03
Locus At1g11170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3744007
Primers used for PCR 17dh - 8474, show primer details
   
Sequence Name 49-K110039-0022-350-E03C-17dh
GenBank Accession LN506872 [GenBank]
Sequencing primer gene specific primer, 17dh: 5'-TTTGTGTGTTGTTGTTGTTCTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K110039-0022-350-E03C-17dh
TGCTTTACTCGGTGACTACTACTACTACCACGCCTTAATCTTCTGTTATTACCGTTACTC
TTCCTCTTTGTCATCGAGCTTGATGACGATGGATCGATCCACTTAATGTCTTCTTCTACC
GCTTTGCTCCATCGTTCTTTAAAAGTTTGAAGCTCCCATGTCGATTGTCTCCTTATCTAC
TTGTTTCATCACAAACACTAGAATGCTAAGTTTTACCGTCTATTGAATACATATCAAAGA
AACAATAATTCATCGGTTAACTCACCTCGGTTCTTGAATCAAAAGTTGTATGGCCTTGTC
TCTTGAAATATCGACAAAAAAAAGAAACACGAAGGAGTGAGTTTTATTTAGTGAAGACCA
AAAGAATTAGAATCGAGAATGTTTTTGAGTCGATACAACTTACCCTGATCATCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 33-K110038-0022-350-E03C-8409
GenBank Accession LN501036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K110038-0022-350-E03C-8409
ATTTACATTGATGATCAGGGTAAGTTGTATCGACTCAAAAACATTCTCGATTCTAATTCT
TTTGGTCTTCACTAAATAAAACTCACTCCTTCGTGTTTCTTTTTTTTGTCGATATTTCAA
GAGACAAGGCCATACAACTTTTGATTCAAGAACCGAGGTGAGTTAACCGATGAATTATTG
TTTCTTTGATATGTATTCAATAGACGGTAAAACTTAGCATTCTAGTGTTTGTGATGAAAC
AAGTAGATAAGGAGACAATCGACATGGGAGCTTCAAACTTTTAAAGAACGATGGAGCAAA
GCGGTAGAAGAAGACATTAAGTGGATCGATCCATCGTCATCAAGCTCGATGACAAAGAGG
AAGAGTAACGGTAATAACAGAAGATTAAGGCGTGGTAGTAGTAGTAGTCACCGAGTAAAG
CATAAGAGAAGTCAAGAGACATCAAGAACAACAACAACACACAA

Last Updated on 06.12.2018 16:11