Sequences from confirmation amplicon


Confirmation sequences for line 351E08 and hit At4g15450 are shown.
Line ID 351E08
Locus At4g15450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8840904
Primers used for PCR GD50 - 8474, show primer details
   
Sequence Name 91-K033713-022-351-E08C-GD50
GenBank Accession LN515084 [GenBank]
Sequencing primer gene specific primer, GD50: 5'-CATGAGGGATAAATTTGGTAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K033713-022-351-E08C-GD50
GAATCAGATTACCATTTGCATATTCTGACTTAATTTTAACCTTATGGTCTCTGCCCACAA
ATAAATACAAACACCAAATATCACTTTCGTGAAGCGTTTTTGAGGATCCCCGATGTGACT
GAAGAGGAAGGAGAGAAAGCTTTGCGGATATTGAAGACGTTCCACGCAGTGCCGGCTGCG
TGCCCAACCGTGCCTAGTACATCTTTCGTAGCTTCTCCCGCGCTCTCACCTAACCTGTTA
ATGTAACCTCACTTATGTGAGACATTTACACTAAACGTGAGGCAAAATTGAGGACTTTGT
CTGTGAAACTTACCTCTCACTAACCATTCTTGTAGTAGCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCCAAATGG
   
Sequence Name 75-K033713-022-351-E08C-8409
GenBank Accession LN513179 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K033713-022-351-E08C-8409
GCCATTTACAATTGCTACTACAGAATGGTTAGTGAGAGGTAAGTTTCACAGACAAAGTCC
TCAATTTTGCCTCACGTTTAGTGTAAATGTCTCACATAAGTGAGGTTACATTAACAGGTT
AGGTGAGAGCGCGGGAGAAGCTACGAAAGATGTACTAGGCACGGTTGGGCACGCAGCCGG
CACTGCGTGGAACGTCTTCAATATCCGCAAAGCTTTCTCTCCTTCCTCTTCAGTCACATC
GGGGATCCTCAAAAACGCTTCACGAAAGTGATATTTGGTGTTTGTATTTATTTGTGGGCA
GAGACCATAAGGTTAAAATTAAGTCAGAATATGCAAATGTGTAATCTTGAATCTCAAACA
AAGGAAACCTCTCTTCCTTACCAAATTTATCCCTCATG

Last Updated on 06.12.2018 16:11