Sequences from confirmation amplicon


Confirmation sequences for line 351H02 and hit At5g55770 are shown.
Line ID 351H02
Locus At5g55770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22573665
Primers used for PCR BV66 - 8474, show primer details
   
Sequence Name 37-K106149-0022-351-H02C-BV66
GenBank Accession LN502621 [GenBank]
Sequencing primer gene specific primer, BV66: 5'-ATCGTCCAGCTAGACTTACGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K106149-0022-351-H02C-BV66
CAGGTCATGAGCATCCCTTGTTCCTACCCTGGGAAACAGAGAAAGAGACACGATGTCAAA
TGTGCAAATACGACAGCGGCCATTCAAAGTTAATTTGCATGGACTGCGATTATTCTATAT
GTTTCCGGTGTGCTACATTCCCATACATGGCAAGGTATAAGCATGACAGTCATTTTCTCA
CAATTTCCAATGGGAAAGAGGAAAGTGATCAGCAAGATTGGTGTGAGATTTGTGAACGCA
AAATAGTAAAAGTAAAAGAAAGAGGGAATGTGCTGTGGAATCAGAAAGAAGCATTGCGAT
ATAGATGCAATGTTTGCTGCACCACTCTTCACATAGATTGTTTACACGGGAAAGAGATGT
ACATCAAACCTCGGGAAACAGAACGAGATCATCTCACTTTTCACACTTTTAGAGACATGG
AAAGGATATTTATTAGAGTCCATCTCAACAGTTCTCTCTCCCGACCCGTTTGCAGTAGAT
GCAATCGTCGTTGTCCATTCCCAATATTTTTCAAAGGATCAGCCAATTAGATGCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCC
   
Sequence Name 53-K106150-0022-351-H02C-8409
GenBank Accession LN508160 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K106150-0022-351-H02C-8409
ATTTACATTGAATATATCCTGCATCTAATTGGCTGATCCTTTGAAAAATATTGGGAATGG
ACAACGACGATTGCATCTACTGCAAACGGGTCGGGAGAGAGAACTGTTGAGATGGACTCT
AATAAATATCCTTTCCATGTCTCTAAAAGTGTGAAAAGTGAGATGATCTCGTTCTGTTTC
CCGAGGTTTGATGTACATCTCTTTCCCGTGTAAACAATCTATGTGAAGAGTGGTGCAGCA
AACATTGCATCTATATCGCAATGCTTCTTTCTGATTCCACAGCACATTCCCTCTTTCTTT
TACTTTTACTATTTTGCGTTCACAAATCTCACACCAATCTTGCTGATCACTTTCCTCTTT
CCCATTGGAAATTGTGAGAAAATGACTGTCATGCTTATACCTTGCCATGTATGGGAATGT
AGCACACCGGAAACATATAGAATAATCGCAGTCCATGCAAATTAACTTTGAATGGCCGCT
GTCGTATTTGCACATTTGACATCGTGTCTCTTTCTCTGTTTCCCAGGGTAGGAACAAGGG
ATGCTCATGACCTTGATAATCAAATGGTTCGATAATCGAGGCACATCGTAAGTC

Last Updated on 06.12.2018 16:11