Sequences from confirmation amplicon


Confirmation sequences for line 352A01 and hit At3g62660 are shown.
Line ID 352A01
Locus At3g62660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23174431
Primers used for PCR GD49 - 8474, show primer details
   
Sequence Name 92-K033713-022-352-A01C-GD49
GenBank Accession LN515158 [GenBank]
Sequencing primer gene specific primer, GD49: 5'-GCGAACTTCACAAAGTATTTCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K033713-022-352-A01C-GD49
TTTCTCCGGGAGGAACCGTGCTACTTCACACGGGAGTGATGGTGATGGATCTAGAGAGAT
GGAGGCGCGTAGGGTACACGGAGGTGATAGAGAAATGGATGGAGATTCAGAAGAGTGATA
GGATTTACGAGCTGGGATCATTGCCGCCGTTCTTGTTGGTGTTCGCCGGAGAAGTAGCTC
CGATAGAGCATCGGTGGAACCAGCATGGGCTTGGTGGAGATAACGTGAGAGGAAGCTGTA
GAGATTTACATCCCGGTCCGGTTAGCTTGCTTCATTGGTCCGGTAGTGGTAAACCGTGGT
TTCGGTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCACGTCCAAATGGG
   
Sequence Name 76-K033713-022-352-A01C-8409
GenBank Accession LN513334 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K033713-022-352-A01C-8409
TTACAATTGAATAAACCGAAACCACGGTTTACCACTACCGGACCAATGAAGCAAGCTAAC
CGGACCGGGATGTAAATCTCTACAGCTTCCTCTCACGTTATCTCCACCAAGCCCATGCTG
GTTCCACCGATGCTCTATCGGAGCTACTTCTCCGGCGAACACCAACAAGAACGGCGGCAA
TGATCCCAGCTCGTAAATCCTATCACTCTTCTGAATCTCCATCCATTTCTCTATCACCTC
CGTGTACCCTACGCGCCTCCATCTCTCTAGATCCATCACCATCACTCCCGTGTTGAAGTA
GCACGGTTTCCTCCCGGAGAAAGCTCCGGAGAACCTCTCGTCGGACCAGAACGCCGGTGT
GAAATACTTTGTGAAGTTCGC

Last Updated on 06.12.2018 16:11