Sequences from confirmation amplicon


Confirmation sequences for line 352C11 and hit At3g11520 are shown.
Line ID 352C11
Locus At3g11520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3625518
Primers used for PCR LB25 - 8474, show primer details
   
Sequence Name 46-K033737-022-352-C11C-8409
GenBank Accession LN505784 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K033737-022-352-C11C-8409
TTCGGACTGAGCCATTTACAATTGAATATATCCTGCAACAGCACCTCTTCCAAGTTTCGA
ATACTTCTTCAACACACCACGTAGCTTGCTCTCCCCCGCTTTCGAGTGAATGAAAGCTAA
AAGCTTTGAGCAATCCCTGATTACACACAGAAACGCACATAGAGAATTAATATACAAACA
ATATGATCACAAACTTGCTAGTGTAAAGAATTAAGAAGACTAACATGAGCTGAGATTCGG
AGTAGCCTGTGTGGAATTTCAGTGTATCAGTCCAAGTAGGAGTCTTGTTCAAGCAACACC
TTGCTGTGTAGACCGCTGATGCAGCAAGCATGGAAGGACAGAACATTAAACTGTCGTGA
   
Sequence Name 48-K033737-022-352-C11C-LB25
GenBank Accession LN506425 [GenBank]
Sequencing primer gene specific primer, LB25: 5'-TCACGACAGTTTAATGTTCTGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K033737-022-352-C11C-LB25
AGCAAGGTGTTGCTTGAACAGACTCCTACTTGGACTGATACACCGAAATTCCACACAGGC
TACTCCGAATCTCAGCTCATGTTACTCTTCTTAATTCTTTACACTAGCAAGTTTGTGATC
ATATTGTTTGTATATTAATTCTCTATGTGCGTTTCTGTGTGTAATCAGGGATTGCTCAAA
GCTTTTAGCTTTCATTCACTCGAAAGCGGGGGAGAGCAAGCTACGTGGTGTGTTGAAGAA
GTATTCGAAACTTGGAAGAGGTGCTGTTGCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11