Sequences from confirmation amplicon


Confirmation sequences for line 352E12 and hit At5g65620 are shown.
Line ID 352E12
Locus At5g65620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26224567
Primers used for PCR 23kh - 8474, show primer details
   
Sequence Name 37-K113531-0022-352-E12C-23kh
GenBank Accession LN502684 [GenBank]
Sequencing primer gene specific primer, 23kh: 5'-ATTCACCTGACGAAGACTAAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K113531-0022-352-E12C-23kh
TTCCTCTGGGAGTGTTTCTCCTGTTTCGTAATGCTTAGCAATGCTCATTAAAGTGTCCCT
GAAACCAGCAATTCATAACGCTCATTTCACATTACTTTGTAAATCACAACGAGGTAACAT
CGATTCTGCCTGGAAATGAACTCCAAATTACCTGTGGTAGCACCAATTCTCCATAAACTG
TGATGGTAATTCAACCGCGTCCCACTCGATATTTCGGATACCAGCAACTAGCCCCTCATC
CTGCTTCGTAAGCATGTGCTGAAGAGCATGTCCAAATTCATGAAATACTGTCTCTACCTG
TAAATCAGAGTGATGTTCATATGTTTTGCACTTGTTTTATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 12-K113530-0022-352-E12C-8409
GenBank Accession LN491658 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K113530-0022-352-E12C-8409
TTCATTGAATAAAACAAGTGCAAACATATGAACATCACTCTGATTTACAGGTAGAGACAG
TATTTCATGAATTTGGACATGCTCTTCAGCACATGCTTACGAAGCAGGATGAGGGGCTAG
TTGCTGGTATCCGAAATATCGAGTGGGACGCGGTTGAATTACCATCACAGTTTATGGAGA
ATTGGTGCTACCACAGGTAATTTGGAGTTCATTTCCAGGCAGAATCGATGTTACCTCGTT
GTGATTTACAAAGTAATGTGAAATGAGCGTTATGAATTGCTGGTTTCAGGGACACTTTAA
TGAGCATTGCTAAGCATTACGAAACAGGAGAAACACTCCCAGAGGAAGTATACAAGAAAC
TCCTGGCCGCAAGAACATTCCGTGCAGGGTCCTTTAGTCTTCTCAAAGTGAATA

Last Updated on 06.12.2018 16:11