Sequences from confirmation amplicon


Confirmation sequences for line 352F03 and hit At3g13920 are shown.
Line ID 352F03
Locus At3g13920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4593134
Primers used for PCR JA34 - 8474, show primer details
   
Sequence Name 42-K100939-0022-352-F03C-8409
GenBank Accession LN504394 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K100939-0022-352-F03C-8409
GTAGCTCATCACGCTTCACCAAGATTCTCACTGGCTTGCTCATGAACTTCCTTGTGATCT
CAAGAGCTTCTGGTGGCATTGTTGCGGAGAACACACCAACTTGGATCTTTGGTGGAAGAA
GCTGGAATATGTCATAGATCTGAAAAAACCCAGAAACCAGTGAGATTCAAGTTAAACTAT
AACGAGAATGGAATTGAAACACTGAGAAATATTTGCATACCTGGTCCTTGAAACCACGGG
AGAGCATTTCATCAGCTTCATCGAGAACAAACATCTTGATGTTGTCAGCACGAAGAGACT
GCCTTCTTCACATGTCAAAGACACGCCCTGGAGTTCCCACCACCACCTGGACCCCAGCTT
GGAAGAAGCGCTTATTCTCAAGGACACTGGTTCCACCCACACAGGCCTGAACCTTGACAC
CAAGGGTATCACCAAGGGCCCTCAAGAACTTCTCAATTTGCTGAACCAGCTCTCTTGT
   
Sequence Name 42-K100946-0022-352-F03C-JA34
GenBank Accession LN504395 [GenBank]
Sequencing primer gene specific primer, JA34: 5'-GACTTCTCCCTTATCCAGTGTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K100946-0022-352-F03C-JA34
CTCAGCAATTGAGAAGGTCATGAGGGCCCTTGGTGATTACCTTGGTGTCAAGGTTCACGC
CTGTGTTGGTGGAACCAGTGTCCGTGAGGATCAGCGCATCCTCCAAGCTGGTGTCCATGT
TGTTGTTGGAACTCCAGGGCGTGTCTTTGACATGTTGAAGAGGCAGTCTCTTCGTGCTGA
CAACATCAAGATGTTTGTTCTCGATGAAGCTGATGAAATGCTCTCCCGTGGTTTCAAGGA
CCAGGTATGCAAATATTTCTCAGTGTTTCAATTCCATTCTCGTTATAGTTTAACTTGAAT
CTCACTGGTTTCTGGGTTTTTT

Last Updated on 06.12.2018 16:11