Sequences from confirmation amplicon


Confirmation sequences for line 353B01 and hit At4g27420 are shown.
Line ID 353B01
Locus At4g27420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13714132
Primers used for PCR 10di - 8474, show primer details
   
Sequence Name 41-K109414-0022-353-B01C-10di
GenBank Accession LN504107 [GenBank]
Sequencing primer gene specific primer, 10di: 5'-CTGTCCAACAGGTTTGTCTTGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K109414-0022-353-B01C-10di
TCTTTGGGATTCATCTGATCCAACACCTATAGAAGTATAATATATCCATGTGAGATTCAA
CACGGAGACACAAAAAACTATAATATGACGGCTTACTTGGCCAGCAAGATATCACAAGGA
GAGGCTTACCGTTTGCGATGTCCAAGAGAAAATCCGAGGGATTAATCCTTTCGACTAAGG
GGGAGTATCCAACGCTAGCAAAATAATCCATAGCATTGGATCCAAGCCCGAAGTAGACCG
GATTACCTTCTGAAAGGAGCAGAAGCTTATCAAACATATAGAACAACCTACTAGAAGGTT
GATGAATTGTAGTCACGACCGTTCTCCCACCACGAGCCAGTTCCCACAAAATCGACACAA
TCCTTTGAGCAGTGGTAGAATCAAGCCCAGAAGTGGGCTCATCAAGAAAGAGCAAACTAG
GGTTTATAAGAATCTCTTGCCCTATACTAACCCTTTTCCTCTCACCACCTGAAACACCTC
TCAAGAAGGGTCCTCCAATGATAGTATCCTTACATCTGTCTAGACCCAGTTCAGTCATGA
CTGCCTTAGCTTGTTTGATCTTCTCTTGTTTTTTAAAAGAATTAGGAAGCCTAAGAAGCG
CAGTGAATACTAGAGTTTCCGTAACAGTCAAGTTAGGGTAAAGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 25-K109413-0022-353-B01C-8409
GenBank Accession LN497483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K109413-0022-353-B01C-8409
CATTTACATTGAATATATCTTTACCCTAACTTGACTGTTACGGAAACTCTAGTATTCACT
GCGCTTCTTAGGCTTCCTAATTCTTTTAAAAAACAAGAGAAGATCAAACAAGCTAAGGCA
GTCATGACTGAACTGGGTCTAGACAGATGTAAGGATACTATCATTGGAGGACCCTTCTTG
AGAGGTGTTTCAGGTGGTGAGAGGAAAAGGGTTAGTATAGGGCAAGAGATTCTTATAAAC
CCTAGTTTGCTCTTTCTTGATGAGCCCACTTCTGGGCTTGATTCTACCACTGCTCAAAGG
ATTGTGTCGATTTTGTGGGAACTGGCTCGTGGTGGGAGAACGGTCGTGACTACAATTCAT
CAACCTTCTAGTAGGTTGTTCTATATGTTTGATAAGCTTCTGCTCCTTTCAGAAGGTAAT
CCGGTCTACTTCGGGCTTGGATCCAATGCTATGGATTATTTTGCTAGCGTTGGATACTCC
CCCTTAGTCGAAAGGATTAATCCCTCGGATTTTCTCTTGGACATCGCAAACGGTAAGCCT
CTCCTTGTGATATCTTGCTGGCCAAGTAAGCCGTCATATTATAGTTTTTTGTGTCTCCGT
GTTGAATCTCACATGGATATATTATACTTCTATAGGTGTTGGATCAGATGAATCCCAAAG
ACCAGAGGCTATGAAGGCAGCTCTAGTTGCGTTTTACAAGACAAACTGNTTGGACA

Last Updated on 06.12.2018 16:11