Sequences from confirmation amplicon


Confirmation sequences for line 353D07 and hit At3g12500 are shown.
Line ID 353D07
Locus At3g12500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3962531
Primers used for PCR 23we - 8474, show primer details
   
Sequence Name 60-K118393-0022-353-D07C-23we
GenBank Accession LN510370 [GenBank]
Sequencing primer gene specific primer, 23we: 5'-TATAGATTGGTTTGATTTGGCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118393-0022-353-D07C-23we
TTCTACCAATCTGGATTACCATGAAAACGTTGCTGAGGATAGCGGCACGTTATACGAAAC
GTGTCGGCAGAGTCACACAGAAATTGTCAAACACCTTTACTTGTAATCGAAGTTTAAAAC
ATCTATGCACCTTTCTTCGTCTTTCTCTCATCTGCTAGAGAGCCCAATTAAAAAAGGCCC
ATGGGAAATAAAATCGCAACATAAACAGTGAAACATCATTGGAAAGAGTTTTATTAGTCT
GATATTATTGTTTTGATAATACAAAGCTGCGTATTATATTATAATAATAATACTCTCGTT
ACTAAATAGCAGCTTCGAGGAGGCCGTTAACGGATCAATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 12-K118392-0022-353-D07C-8409
GenBank Accession LN491794 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118392-0022-353-D07C-8409
ATTTACATTGAAATATTGATCCGTTAACGGCCTCCTCGAAGCTGCTATTTAGTAACGAGA
GTATTATTATTATAATATAATACGCAGNCTTTGTATTATCAAAACAATAATATCAGACTA
ATAAAACTCTTTCCAATGATGTTTCACTGTTTATGTTGCGATTTTATTTCCCATGGGCCT
TTTTTAATTGGGCTCTCTAGCAGATGAGAGAAAGACGAAGAAAGGTGCATAGATGTTTTA
AACTTCGATTACAAGTAAAGGTGTTTGACAATTTCTGTGTGACTCTGCCGACACGTTTCG
TATAACGTGCCGCTATCCTCAGCAACGTTTTCATGTGTAATCCAGATTGGTATGAATTTC
TATTTAAAATGTTGGTAGCTTAACCGATTGAGGCCAAATCAACCCAAATCTAT

Last Updated on 06.12.2018 16:11