Sequences from confirmation amplicon


Confirmation sequences for line 353F05 and hit At5g05250 are shown.
Line ID 353F05
Locus At5g05250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1557179
Primers used for PCR BU20 - 8474, show primer details
   
Sequence Name 06-K104372-0022-353-F05C-BU20
GenBank Accession LN488795 [GenBank]
Sequencing primer gene specific primer, BU20: 5'-GAGAAACAGAGAGGTGGAAGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K104372-0022-353-F05C-BU20
GTTGGGAGTGGAGCGGAGCGTGAGAAGCTGACAGGTGGATGAAGGAATCGGTGACGGAGA
TCGTTAAGAATTTAAGTGAAGCGCCGTTGTTAGTCCATTTGTACACCGGAGATAAGGAGG
AGGGAACGGTAGTTGTGATGAAAGCAGAGGAATGGGCGGCGGTGAAGGGGAGGTGGGAGA
GAGGAGAGGCGGAGATGCCGGAAGGGATAGTGTTCGTGGAGCAGTTAGGAGCGGCGGAGG
AGAGTTGTGGCTGTGGTTTTGACGGTGGAGACGGCACACGTGCGTGGGGGTTGGTGGTGC
AAGGGAGAGGAGTTGAGTGTGGGCCGGTTTGTTATCTGCTTAAGACGACCCGTGTAGGGT
CAGGGTCAGGATCCGGGTCGGGTTTGGGTATGCGGTGTACGCATTTCTGTTTAGCTAAGA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGACCGC
ACGNTTATT
   
Sequence Name 22-K104373-0022-353-F05C-8409
GenBank Accession LN496096 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K104373-0022-353-F05C-8409
CATTTAAATCTTAGCTAAACAGAAATGCGTACACCGCATACCCAAACCCGACCCGGATCC
TGACCCTGACCCTACACGGGTCGTCTTAAGCAGATAACAAACCGGCCCACACTCAACTCC
TCTCCCTTGCACCACCAACCCCCACGCACGTGTGCCGTCTCCACCGTCAAAACCACAGCC
ACAACTCTCCTCCGCCGCTCCTAACTGCTCCACGAACACTATCCCTTCCGGCATCTCCGC
CTCTCCTCTCTCCCACCTCCCCTTCACCGCCGCCCATTCCTCTGCTTTCATCACAACTAC
CGTTCCCTCCTCCTTATCTCCGGTGTACAAATGGACTAACAACGGCGCTTCACTTAAATT
CTTAACGATCTCCGTCACCGATTCCTTCATCCACCTGTCTAGCTTCTCACGCTCCGCTTC
CACCTCCCAACGATCTCCTCTGCTTCTCCCTCCGCTTCTTCTTCCACCTCTCTGTTTC

Last Updated on 06.12.2018 16:11